HIV感染对外周血肠道归巢Tc1/Treg、Tc17/Treg平衡的影响

新发传染病电子杂志 ›› 2017, Vol. 2 ›› Issue (2): 76-80.

HIV感染对外周血肠道归巢Tc1/Treg、Tc17/Treg平衡的影响

彭巧丽¹, 程林², 唐娴², 蒋强², 肖金凤¹, 何云¹, 王辉¹

1.深圳市第三人民医院感染一科,广东深圳 518112;
2.深圳市第三人民医院肝病研究所,广东深圳 518112

收稿日期:2016-04-06 出版日期:2017-05-30 发布日期:2020-07-01
通讯作者: 王辉,Email：huiwang98@yahoo.com
基金资助:
国家自然科学基金青年基金项目（31500750）; “十二五”科技重大专项子课题（2012ZX10001006-001-009）; 深圳市科技创新委基础研究计划（JCYJ20150402111430627）; 深圳市科技创新委基础研究计划（JCYJ20160427183909738）

The influence of HIV infection on the balance of peripheral gut-homing Tc1/Treg and Tc17/Treg

PENG Qiao-li¹, CHEN Lin², TANG Xian², JIANG Qiang², XIAO Jin-feng¹, HE Yun¹, WANG Hui¹

1. The Department of Infectious disease,Shenzhen Third People′s Hospital,Guangdong Shenzhen 518112,China;
2. The Institute of Hepatology, Shenzhen Third People′s Hospital,Guangdong Shenzhen 518112,China

Received:2016-04-06 Online:2017-05-30 Published:2020-07-01

摘要/Abstract

摘要： 目的探讨艾滋病病毒（HIV）感染对外周血肠道归巢CD8+T细胞亚群Tc1、Tc17与肠道归巢Treg平衡的影响。方法选取62例符合诊断标准的无症状HIV感染者：未治疗组31例,治疗组31例,同时选取41例健康对照,采用流式细胞表面及全血胞内细胞因子染色方法,使用BD FACSCanto流式细胞仪检测各项指标,FACSDiva软件分析肠道归巢CD8+T细胞亚群Tc1、Tc17与肠道归巢CD4+T细胞亚群Treg之间的比值变化,比较各组之间的差异及与CD4计数、病毒载量之间的相关性。结果 HIV感染后外周血肠道归巢Tc1/Treg比值显著增高（24.68±16.12 vs 11.57±4.75,P<0.001）,抗病毒治疗可使该比值显著降低至健康对照水平（15.43±13.90 vs 11.57±4.75,P=0.617）;而外周血肠道归巢Tc17/Treg比值在HIV感染后则显著降低（0.61±2.44 vs 1.02±0.95,P<0.001）,抗病毒治疗不能恢复该比值（0.57±0.78 vs 0.61±2.44,P<0.001）,仍显著低于健康对照（0.57±0.78 vs 1.02±0.95,P<0.001）;相关性分析表明,外周血肠道归巢Tc1/Treg比值与病毒载量负相关（r=-0.633,P<0.001）,与CD4计数无相关性,而Tc17/Treg比值则与病毒载量负相关（r=-0.387,P=0.034）,与CD4计数正相关（r=0.404,P=0.027）。结论 HIV感染导致外周血肠道归巢T细胞亚群Tc1、Tc17与Treg平衡紊乱,且与疾病进展相关,抗病毒治疗不能完全修复这种平衡失调。

关键词: 艾滋病病毒, 肠道归巢T细胞, 辅助细胞1, 辅助细胞17, 调节性T细胞

Abstract: Objective To investigate the influence of HIV infection on the balance of peripheral gut-homing CD8+T cell Tc1/Treg and Tc17/Treg. Methods 62 asymptomatic HIV infected patients as well as 41 healthy volunteers were recruited in this study. The patients were divided into two groups, which were the untreated and the treated group. The whole blood surface and intracellular cytokine staining were detected by BD FACSCanto, after that, the expression of gut-homing CD8+T cell subpopulation Tc1, Tc17 and Treg were analyzed by FACSDiva software and compared the differences of Tc1/Treg and Tc17/Treg ratio among different groups and the correlation with CD4 count and viral load. Results The ratio of peripheral gut homing Tc1/Treg increased significantly after HIV infection (24.68+16.12 vs 11.57±4.75,P<0.001), ART treatment can bring the ratio to the nearly normal level（15.43+13.90 vs 11.57±4.75,P=0.617）. Meanwhile, the ratio of peripheral gut homing Tc17/Treg showed a significant decrease（0.61±2.44 vs 1.02±0.95,P<0.001）. ART can not restore this ratio to normal (0.57±0.78 vs 0.61±2.44, P<0.001),which was still much lower than healthy control（0.57±0.78 vs 1.02±0.95,P<0.001）.The correlation analysis showed that the ratio of peripheral gut-homing Tc1/Treg was negatively correlated with viral load（r=-0.633,P<0.001）, but no relationship with CD4 count was found. Different to Tc1/Treg, the ratio of gut-homing Tc17/Treg were negatively correlated with viral load （r=-0.387,P=0.034）and positively with CD4 count（r=0.404,P=0.027）. Conclusion The peripheral gut-homing T cell functional subpopulation Tc1, Tc17 and Treg break homeostasis in vivo after HIV infection, which is correlated with disease progression. Antiretroviral therapy can not reconstitute this imbalances completely.

Key words: HIV, Gut-homing T cell, Tc1, Tc17, Treg

彭巧丽, 程林, 唐娴, 蒋强, 肖金凤, 何云, 王辉. HIV感染对外周血肠道归巢Tc1/Treg、Tc17/Treg平衡的影响[J]. 新发传染病电子杂志, 2017, 2(2): 76-80.

PENG Qiao-li, CHEN Lin, TANG Xian, JIANG Qiang, XIAO Jin-feng, HE Yun, WANG Hui. The influence of HIV infection on the balance of peripheral gut-homing Tc1/Treg and Tc17/Treg[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(2): 76-80.

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