丙泊酚和氯胺酮对结核病小鼠术后T淋巴细胞亚群和结核杆菌复制能力的影响

新发传染病电子杂志 ›› 2017, Vol. 2 ›› Issue (2): 89-92.

丙泊酚和氯胺酮对结核病小鼠术后T淋巴细胞亚群和结核杆菌复制能力的影响

刘民强, 韩亚坤, 余德慧, 马明飞, 石浩文, 何仁亮

深圳市第三人民医院麻醉科,广东深圳 518112

收稿日期:2016-02-17 出版日期:2017-05-30 发布日期:2020-07-01
通讯作者: 何仁亮,E-mail：szherenliang@163.com
基金资助:
广东省深圳市科技知识创新计划项目（JCYJ20140411112047879）

Influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice

LIU Min-qiang, HAN Ya-kun, YU De-hui, MA Ming-fei, SHI Hao-wen, HE Ren-liang

Department of Anesthesiology,The Third People's Hospital of ShenZhen,ShenZhen 518112,China

Received:2016-02-17 Online:2017-05-30 Published:2020-07-01

摘要/Abstract

摘要： 目的比较丙泊酚和氯胺酮对结核病小鼠术后T淋巴细胞亚群和结核杆菌复制能力的影响。方法建立实验小鼠结核病模型,选取实验小鼠36只,随机分为3组：P组、K组和C组,每组12只。P组、K组和C组分别施以麻醉药丙泊酚和氯胺酮及生理盐水。然后对3组小鼠行腹腔切开并对肠管施行牵拉刺激术。分别在术前30分钟、术后1天、术后3天抽取小鼠尾静脉血。利用流式细胞仪检测血液样本中CD3+、CD4+细胞、CD4+/CD8+细胞比值及γ-干扰素（IFN-γ）的表达。术后第3周、6周每组各取6只小鼠,处死后取肺组织进行病理形态学染色及结核菌培养实验。结果与C组相比,P组及K组术后CD3+细胞、CD4+、CD4+/CD8+T细胞比值及IFN-γ表达下降,肺内结核菌定植数增高（P<0.05）。与K组比较,P组术后3天的CD3+、CD4+T细胞水平较高,IFN-γ含量较低（P<0.05）,但上述两组对肺内结核菌复制的影响无明显差异（P>0.05）。结论丙泊酚和氯胺酮均可抑制结核病小鼠术后T淋巴细胞功能,肺内结核菌定植数增高,但两者对T淋巴细胞亚群及IFN-γ的抑制不同步,尚需进一步开展相关研究。

关键词: 丙泊酚, 氯胺酮, 结核杆菌, 小鼠, T淋巴细胞亚群

Abstract: Objective To compare the influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice. Methods 36 mice were enrolled in this study, and were randomly divided into propofol group (group P), ketamine group (group K), and control group (group C) with 12 cases each. Tuberculosis model was established in all mice 6 weeks before the test. Blood samples were taken from the tail vein at 30 minutes before operation, 1 day after the operation and 3 days post-operation, CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD4⁺/CD8⁺T lymphocyte ratio, and interferon gamma (IFN-γ) expression were calculated with flow cytometer. Lung slices and tubercle bacillus culture were observed at 3 weeks and 6 weeks after surgery with 6 mice in each group. Results Compared with group C, CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD4⁺/CD8⁺T lymphocyte ratio, and IFN-γ expression in group P and group K decreased apparently after surgery, and tuberculosis bacterium count was higher (P<0.05). Compared with group K, CD3⁺T lymphocyte, CD4⁺T lymphocyte levels were higher but IFN-γ lower 3 days postoperative in group P (P <0.05), but there was no difference about postoperative tubercle bacillus replication in these two groups. Conclusion Both propofol and ketamine could inhibit postoperative T lymphocyte's function in tuberculosis mice, and tuberculosis bacterium number increase. However, the influence on T lymphocyte subtypes were not synchronously, meaning further researches are needed.

Key words: Propofol, Ketamine, Tubercle bacillus, Mice, T lymphocyte subtype

刘民强, 韩亚坤, 余德慧, 马明飞, 石浩文, 何仁亮. 丙泊酚和氯胺酮对结核病小鼠术后T淋巴细胞亚群和结核杆菌复制能力的影响[J]. 新发传染病电子杂志, 2017, 2(2): 89-92.

LIU Min-qiang, HAN Ya-kun, YU De-hui, MA Ming-fei, SHI Hao-wen, HE Ren-liang. Influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(2): 89-92.

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