广泛耐药结核分枝杆菌对利奈唑胺的耐药情况分析

新发传染病电子杂志 ›› 2017, Vol. 2 ›› Issue (3): 160-163.

• 结核病基础及诊治前沿技术专题 • 上一篇下一篇

广泛耐药结核分枝杆菌对利奈唑胺的耐药情况分析

宗兆婧, 荆玮, 霍凤敏, 尚媛媛, 梁倩, 李云絮, 逄宇, 黄海荣

首都医科大学附属北京胸科医院,北京市结核病胸部肿瘤研究所,国家结核病临床实验室北京 101149

收稿日期:2017-06-13 出版日期:2017-08-31 发布日期:2020-07-13
通讯作者: 逄宇,Email: pangyupound@163.com
基金资助:
北京市通州区科技计划（KJ2017CX076）

Resistance analysis of extensively drug-resistant mycobacterium tuberculosis isolatesagainst linezolid

ZONG Zhao-jing, JING Wei, HUO Feng-min, SHANG Yuan-yuan, LIANG Qian, LI Yun-xu, PANG Yu, HUANG Hai-rong

National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, 101149,China

Received:2017-06-13 Online:2017-08-31 Published:2020-07-13

摘要/Abstract

摘要： 目的分析广泛耐药结核分枝杆菌临床菌株对利奈唑胺的耐药特征,探讨可能的耐药机制。方法选取2012年9月至2014年4月在首都医科大学附属北京胸科医院住院治疗患者的90株临床分离菌株,应用微孔板Alamar blue染色法进行体外药敏试验,并对所有菌株中利奈唑胺耐药相关基因rplC、rplD和23S rRNA的序列进行测定。结果 90株广泛耐药结核分枝杆菌对常见二线抗结核药物耐药率高。在体外药敏检测中以1μg/ml作为耐药判读标准,其耐药发生率低(5/90,5.6%)。且发生利奈唑胺耐药相关基因突变的菌株仅有2个(2/90,2.2%),耐药相关基因突变和耐药表型无明显相关性。结论利奈唑胺耐药相关基因突变率低,且与体外耐药表型无明显相关性,该药物独特的耐药机制不易与已知抗结核药物发生交叉耐药,在用于广泛耐药结核病的治疗中有明显优势。

关键词: 广泛耐药结核病, 利奈唑胺, 微生物敏感性试验, 多药耐药相关蛋白质类, 点突变

Abstract: Objective To investigate the resistant characteristic of extensively drug-resistant Mycobacterium tuberculosis isolatesagainst linezolid (Lzd). Methods Ninety extensively drug-resistant Mycobacterium tuberculosis clinical isolates were collected from Beijing Chest Hospital during 2012-2014. Broth dilution micro-plate alamar blue assay (MABA) was used to detect the minimal inhibitory concentration(MIC) of isolates against Lzd. Lnz resistant genes rplC、rplDand 23S rRNA were sequenced for all the enrolled isolates. Results Total of ninety isolates were enrolled .The resistant rate to common second-line anti-tuberculosis drugs is high while only 5(5/90, 5.6%) of these isolates resistant to Lzd. Mutations at 154 locus of rplC were detected in two of the isolates, and Cys→Arg has no obvious accounting to Lzd resistance. All the strains had no mutations in rplD and 23S rRNA. Conclusion The study demonstrated that linezolid resistance and mutation were rarely among extensively drug-resistant Mycobacterium tuberculosisisolates. This drug is of great use in therapy of extensively drug-resistant tuberculosis.

Key words: Extensively drug-resistant tuberculosis, Linezolid, Microbial sensitivity tests, Multidrug resistance-associated proteins, Point Mutation

宗兆婧, 荆玮, 霍凤敏, 尚媛媛, 梁倩, 李云絮, 逄宇, 黄海荣. 广泛耐药结核分枝杆菌对利奈唑胺的耐药情况分析[J]. 新发传染病电子杂志, 2017, 2(3): 160-163.

ZONG Zhao-jing, JING Wei, HUO Feng-min, SHANG Yuan-yuan, LIANG Qian, LI Yun-xu, PANG Yu, HUANG Hai-rong. Resistance analysis of extensively drug-resistant mycobacterium tuberculosis isolatesagainst linezolid[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(3): 160-163.

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广泛耐药结核分枝杆菌对利奈唑胺的耐药情况分析

Resistance analysis of extensively drug-resistant mycobacterium tuberculosis isolatesagainst linezolid

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