Elispot检测抗原特异性IFN-γ反应在结核性盆腔炎诊断中的应用价值

新发传染病电子杂志 ›› 2017, Vol. 2 ›› Issue (3): 167-169.

Elispot检测抗原特异性IFN-γ反应在结核性盆腔炎诊断中的应用价值

刘玉娟, 蔡秋娥, 段艳辉, 张明霞, 杨倩婷, 邓群益

深圳市第三人民医院,广东深圳,518112

收稿日期:2017-03-11 出版日期:2017-08-31 发布日期:2020-07-13
通讯作者: 邓群益,Email: dengqunyi@hotmail.com
基金资助:
深圳市科技计划项目(CXZZ20140411112047886、JCYJ20150402111430653); 深圳市卫生计生系统科研项目(201501029)

Application values of antigen specific-IFN-γresponses in tuberculosis pelvic inflammation patients using Elispot

LIU Yu-juan, CAI Qiu-e, DUAN Yan-hui, ZHANG Ming-xia, YANG Qian-ting DENG Qun-yi

Shenzhen Third People's Hospital, Guangdong Shenzhen 518112, China

Received:2017-03-11 Online:2017-08-31 Published:2020-07-13

摘要/Abstract

摘要： 目的评价酶联免疫斑点(Elispot)检测外周血中结核分枝杆菌性抗原特异性γ干扰素（IFN-γ）水平在结核性盆腔炎诊断中的价值。方法利用Elispot技术分别对结核性盆腔炎患者（63例）和非结核性盆腔炎（19例）外周血中产生结核菌抗原特异性IFN-γ的水平进行定量检测。结果结核组中IFN-γ表达水平是94.40±9.210,非结核组是11.79±3.127,两者间有统计学差异（P<0.05）;结核组阳性率是80.9%,非结核组是10.5%。结论 Elispot检测可提高结核性盆腔炎的诊断率,对结核性盆腔炎诊断提供很好的依据,是结核性盆腔炎较好的实验室诊断手段,具有较高的应用价值和广阔的应用前景。

关键词: 结核性盆腔炎, 免疫酶联免疫斑点, γ干扰素

Abstract: Objective To investigate mycobacterium tuberculosis antigen specific IFN-γ response in tuberculosis pelvic inflammation patients（TPIP）by Elispot. Methods The levels of IFN-γ production in response to mycobacterium tuberculosis antigens in TPIP (n=63) and non-TPIP(n=19) were evaluated by an in-house Elispot. Result By using Elispot, the level of IFN-γin TPIP was much higher than the non-TPIP ,and there was statistical difference between the TPIP and the non-TPIP(p<0.05).The positive rate in Elispot observed in TPIP and non-TPIP was 80.9%,10.5% respectively. Conclusion It is practical to differentiate the tuberculosis pelvic inflammation patients from the non tuberculosis pelvic inflammation patients by measuring the Mtb. antigen-specific IFN-γ response in the peripheral blood using our in-house Elispot assay. It is reliable to use it to diagnosis the TPIP.

Key words: Tuberculosis pelvic inflammation, Elispot, Interferon-gamma

刘玉娟, 蔡秋娥, 段艳辉, 张明霞, 杨倩婷, 邓群益. Elispot检测抗原特异性IFN-γ反应在结核性盆腔炎诊断中的应用价值[J]. 新发传染病电子杂志, 2017, 2(3): 167-169.

LIU Yu-juan, CAI Qiu-e, DUAN Yan-hui, ZHANG Ming-xia, YANG Qian-ting DENG Qun-yi. Application values of antigen specific-IFN-γresponses in tuberculosis pelvic inflammation patients using Elispot[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(3): 167-169.