IP-10在结核患者中的表达及在诊断中的应用

新发传染病电子杂志 ›› 2017, Vol. 2 ›› Issue (4): 198-201.

IP-10在结核患者中的表达及在诊断中的应用

杨倩婷^1,*, 邓国防^1,*, 尤信信², 陈骑¹, 张明霞¹, 邓群益¹, 周一平³

1.深圳市第三人民医院,广东省新发传染病诊治重点实验室,广东深圳 518020;
2.广东医科大学,广东湛江 524003;
3.深圳市福田区人民医院,广东深圳 518112

收稿日期:2017-01-05 出版日期:2017-11-30 发布日期:2020-07-14
通讯作者: 周一平,Email：yqt2010@hotmail.com
作者简介:*为并列第一作者
基金资助:
国家自然科学基金面上项目（81671984）,广东省自然科学基金项目（2015A030313879）; 深圳市科技计划项目（JCYJ20160427184123851,JCYJ20150402111430656,JCYJ20160427151540695,JCYJ20150402145015986 ）

Eexpression of IP-10 in patients with tuberculosis and its value in diagnosis of tuberculosis

YANG Qian-ting^1,*, DENG Guo-fang^1,*, YOU Xin-xin², CHEN Qi¹, ZHANG Ming-xia¹, DENG Qun-yi¹, ZHOU Yi-ping³

1. Third People's Hospital of ShenZhen, Guang dong Key Laboratory for Emerging infectious Disease, Guangdong Shenzhen 518020, China;
2. Guangdong Medical University, Guangdong Zhanjiang 524003, China;
3. Shenzhen Futian District people's Hospital,Guangdong Shenzhen 518112, China

Received:2017-01-05 Online:2017-11-30 Published:2020-07-14

摘要/Abstract

摘要： 目的研究全血干扰素诱导蛋白10（interferon-inducible protein10,IP-10）在不同人群不同标本中的表达情况,评价其在结核病诊断中的应用价值。方法采用免疫酶联吸附试验（ELISA）法来检测24例健康人、24例结核潜伏患者及34例结核患者血浆中的IP-10表达量,并对结核病患者进行随访,观察抗结核治疗后IP-10表达的变化。检测23例结核患者和15例非结核患者脑脊液中IP-10表达量,通过绘制ROC曲线,评价IP-10对结核病的诊断效能。结果结核患者血浆中IP-10显著高于健康人和结核潜伏患者;IP-10在分辨结核病人和健康对照或潜伏感染者有较好的效果,AUC均在0.9以上;结核患者血浆中IP-10随着治疗的进行逐渐降低;结核患者脑脊液和胸腔积液中IP-10显著高于非结核患者。结论 IP-10在结核患者中高表达,能有效地区分结核病,可用于诊断结核的生物标识。

关键词: IP-10, 结核病, 诊断, 生物标识

Abstract: Objective To analyze the expression of interferon-inducible protein 10 (IP-10) in whole blood of different group of people and different type of specimens and evaluate its value in diagnosis of tuberculosis. Methods Immune enzyme-linked adsorption test (ELISA) was employed to detect IP-10 expression in plasma of 24 cases of healthy control (HC), 24 cases of latent tuberculosis（LTBI）and 34 cases of patients with tuberculosis (TB), to detect IP-10 expression in pleural fluid of 23 patients with tuberculosis and 15 cases of non-tuberculosis, and to detect IP-10 expression in cerebrospinal fluid of 23 patients with tuberculosis and 15 cases of non-tuberculosis. Subsequently, ROC curve was drawn to evaluate the value of IP-10 in diagnosis of TB. Results The level of IP-10 in plasma of TB patients was significantly higher than those of HC and LTBI. The IP-10 level in plasma of TB gradually reduced after anti-TB treatment. The level of IP-10 in cerebrospinal fluid and pleural effusion of TB were significantly higher than those in the patients with non-tuberculosis. Conclusion IP-10 could be used as a biomarker in diagnosis of TB.

Key words: IP-10, Tuberculosis, Diagnosis, Biomarker

杨倩婷, 邓国防, 尤信信, 陈骑, 张明霞, 邓群益, 周一平. IP-10在结核患者中的表达及在诊断中的应用[J]. 新发传染病电子杂志, 2017, 2(4): 198-201.

YANG Qian-ting, DENG Guo-fang, YOU Xin-xin, CHEN Qi, ZHANG Ming-xia, DENG Qun-yi, ZHOU Yi-ping. Eexpression of IP-10 in patients with tuberculosis and its value in diagnosis of tuberculosis[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(4): 198-201.

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