关键词: γ干扰素释放试验;, 结核性盆腔炎, 高通量抗体芯片技术, 诊断

Abstract: Objective To explore the significance and application value of interferon-γ release assays(IGRA) in the diagnosis of early pelvic tuberculosis.Methods 46 cases of pelvic tuberculosis patients diagnosed in the Third People’s Hospital of Shenzhen from June 2014 to June 2017 were selected as experimental group and randomly 30 cases of non-tuberculous pelvic were selected as control group. The pelvic effusion which was extracted by laparoscopic surgery or abdominal puncture and the peripheral blood of all patients were detected by using tuberculous specific IFN-γ ELISPOT detection techniques(IGRA combining high-throughput antibody chip technology) to evaluate the specificity and sensitivity of the techniques.Result sThe positive diagnosis rate of pelvic effusion in the experimental group was 97.8%(45/46), 87.0%(40/46) in peripheral blood, and the positive diagnosis rate of pelvic effusion was significantly higher than that of peripheral blood specimens (P<0.01). The positive rate of diagnosis in patients with pelvic tuberculosis was 97.8%(45/46) and 10.0%(3/30) in patients of control group. The diagnostic sensitivity and specificity of tuberculous specific IFN-γ ELISPOT test were more than 85%.Conclusion The tuberculous specific IFN-γ ELISPOT detection technique with highly specific and sensitive to early pelvic tuberculosis can specifically distinguish pelvic tuberculosis from non-tuberculous pelvic inflammation, which is worth clinical promotion.

Key words: Interferon-γ release assays(IGRA);, Pelvic tuberculosis, High-throughput antibody chip technology, Diagnosis