1株登革Ⅰ型病毒的分离鉴定及系统进化分析

新发传染病电子杂志 ›› 2020, Vol. 5 ›› Issue (2): 100-103.

1株登革Ⅰ型病毒的分离鉴定及系统进化分析

王宇航¹, 张星艳¹, 花群义2, 任琴¹, 戴少波¹, 李体远¹, 金宁一³, 周伯平¹, 吴诗品¹, 李昌³, 杜寿文¹

1.暨南大学第二临床医学院、深圳市人民医院感染内科,广东深圳 518020;
2.深圳海关动植物检验检疫技术中心,广东深圳 518000;
3.军事科学院军事医学研究院军事兽医研究所、省部共建吉林省人兽共患病预防与控制重点实验室,长春 130062

收稿日期:2020-02-21 出版日期:2020-02-20 发布日期:2020-06-19
通讯作者: 杜寿文,Email：du-guhong@163.com;李昌,Email：lichang78@163.com
基金资助:
1. 国家自然科学基金（31702210，31972719）；
2. 深圳市政府卫生“三名工程”专项基金(SZSM201612034)

Isolation, identification and phylogenetic analysis of a dengue virus I

Wang Yuhang¹, Zhang Xingyan¹, Hua Qunyi², Ren Qin¹, Dai Shaobo¹, Li Tiyuan¹, Jin Ningyi³, Zhou Boping¹, Wu Shipin¹, Li Chang³, Du Shouwen¹

1. Department of Infectious Diseases, the Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Guangdong Shenzhen 518020, China;
2. Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Customs District P.R.C., Guangdong Shenzhen 518000, China;
3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130062, China

Received:2020-02-21 Online:2020-02-20 Published:2020-06-19

摘要/Abstract

摘要： 目的了解1株登革病毒分离株的来源及进化情况。方法分离获得1株登革病毒,通过对该毒株进行血清型分型鉴定及基因组序列测定,进而通过生物信息学方法对囊膜蛋白E进行系统进化分析。结果分离获得1株DENV血清Ⅰ型基因Ⅰ型毒株;且E基因的同源性分析显示,与Malaysia 0802aTw、Singapore株、浙江17-45株、YN176282株、BNJH17108株的核苷酸同源性均在99%以上;氨基酸水平,与NJX-14株、浙江17-45株、Malaysia 0802aTw株、1208aTw株等多种毒株的E蛋白的同源性均为99.8%, 表明这些毒株可能源于同一株原始毒株,且E蛋白序列遗传进化稳定,与2002年以来多株广东省分离的毒株高度同源。结论近十年发现在我国的DENVⅠ型基因Ⅰ型遗传进化稳定,保守程度高,有利于针对该血清型登革病毒的防控。

关键词: 登革病毒, 基因型, 系统进化

Abstract: Objective To preliminarily understand the origin and evolution of the dengue virus isolated strain. Methods A dengue virus was isolated, and its serotyping and genome sequence was identified and analyzed. Especially, the phylogenetic analysis of envelope protein E was performed by bioinformatics methods, ResultsA serotype Ⅰof DENV isolated strain named as SZ905 was identified to be genotype Ⅰ. And the homology analysis of E gene showed that the nucleotide homology with Malaysia 0802atw strain, Singapore strain, Zhejiang 17-45 strain, YN176282 strain and BNJH 17108 strain were all above 99%, and the homology of E protein with the multiple strains such as NJX-14, Zhejiang 17-45, Malaysia 0802atw and 1208atw strains was all above 99.8% in amino acid level, indicating that these strains maybe originate from a same original strain. And the evolution of E protein sequence is stable and highly homologous with that of several strains isolated in Guangdong Province since 2002. Conclusion DENV-Ⅰ genotype Ⅰ in China has stable genetic evolution and high degree of conservation, which is conducive to the prevention and control of this serotype virus disease in recent years.

Key words: Dengue virus, Genotype, Phylogenetic analysis

王宇航, 张星艳, 花群义2, 任琴, 戴少波, 李体远, 金宁一, 周伯平, 吴诗品, 李昌, 杜寿文. 1株登革Ⅰ型病毒的分离鉴定及系统进化分析[J]. 新发传染病电子杂志, 2020, 5(2): 100-103.

Wang Yuhang, Zhang Xingyan, Hua Qunyi, Ren Qin, Dai Shaobo, Li Tiyuan, Jin Ningyi, Zhou Boping, Wu Shipin, Li Chang, Du Shouwen. Isolation, identification and phylogenetic analysis of a dengue virus I[J]. Electronic Journal of Emerging Infectious Diseases, 2020, 5(2): 100-103.

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