siRNA下调KMT2E表达对登革Ⅱ型病毒增殖的影响

新发传染病电子杂志 ›› 2020, Vol. 5 ›› Issue (2): 104-106.

siRNA下调KMT2E表达对登革Ⅱ型病毒增殖的影响

林吉辉¹, 杜寿文¹, 李昌², 花群义³, 吴诗品¹

1.暨南大学第二临床医学院、深圳市人民医院感染内科,广东深圳 518020;
3.军事科学院军事医学研究院军事兽医研究所、省部共建吉林省人兽共患病预防与控制重点实验室,长春 130062;
2.深圳海关动植物检验检疫技术中心,广东深圳 518000

收稿日期:2020-02-12 出版日期:2020-02-20 发布日期:2020-06-19
通讯作者: 杜寿文,Email:du-guhong@163.com;吴诗品,Email:wupoem@126.com
基金资助:
1. 国家自然科学基金（31702210）；
2. 深圳市政府卫生“三名工程”专项基金(SZSM201612034)；
3.兽医生物技术国家重点实验室开放课题（SKLVBF201914）

The effect of KMT2E knockdown with siRNA on the proliferation of dengue virus Ⅱ

Lin Jihui¹, Du Shouwen¹, Li Chang², Hua Qunyi³, Wu Shipin¹

1. Department of Infectious Diseases, the Second Clinical Medical College, Shenzhen People's Hospital of Jinan University, Guangdong Shenzhen 518020, China;
2. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun 130062, China;
3. Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Customs District P.R.C., Guangdong Shenzhen 518000, China

Received:2020-02-12 Online:2020-02-20 Published:2020-06-19

摘要/Abstract

摘要： 目的探究下调赖氨酸甲基转移酶2（lysine methyltransferase 2E,KMT2E）蛋白表达对登革Ⅱ型病毒（dengue virus Ⅱ,DENVⅡ）增殖的影响,初步分析KMT2E在DENVⅡ增殖过程中的调控作用。方法针对KMT2E的编码序列设计3条小干扰RNA（small interfering RNA,siRNA）,转染293T细胞以筛选出下调KMT2E表达效率最高的siRNA,并用实时荧光定量分析DENVⅡ在KMT2E表达下调后的增殖情况。结果编号为1的siRNA（siKMT2E-1）有最高的下调KMT2E蛋白表达效率,转染293T细胞后能明显抑制DENVⅡ增殖。结论 KMT2E可能在DENVⅡ增殖过程中发挥正调控作用。

关键词: 登革Ⅱ型病毒;, 赖氨酸甲基转移酶2E, 小干扰RNA

Abstract: Objective To explore the effect of lysine methyltransferase 2E (KMT2E) knockdown on DENV virus Ⅱ (DENVⅡ) proliferation and preliminarily analyze the role of KMT2E in DENVⅡ proliferation. MethodAccording to the coding sequence of the kmt2e gene, three siRNAs were designed. 293T cells were transfected with these siRNAs to select the one with the highest efficiency in knockdown of KMT2E. RT-qPCR was used to analyze the proliferation of DENVⅡ after knockdown of KMT2E. ResultThe siRNA numbered as siKMT2E-1 possesses the highest knockdown efficiency of KMT2E protein, and can significantly inhibit DENVⅡ proliferation once transfected to 293T cells. Conclusion KMT2E maybe plays a positive role in the proliferation of DENVⅡ.

Key words: Dengue virus Ⅱ, Lysine methyltransferase 2E, Small interfering RNA

林吉辉, 杜寿文, 李昌, 花群义, 吴诗品. siRNA下调KMT2E表达对登革Ⅱ型病毒增殖的影响[J]. 新发传染病电子杂志, 2020, 5(2): 104-106.

Lin Jihui, Du Shouwen, Li Chang, Hua Qunyi, Wu Shipin. The effect of KMT2E knockdown with siRNA on the proliferation of dengue virus Ⅱ[J]. Electronic Journal of Emerging Infectious Diseases, 2020, 5(2): 104-106.