HIV分子流行病学研究和实践进展

摘要/Abstract

摘要： 本文系统地综述了近年来国内外艾滋病分子流行病学研究和实践方面的进展, 主要从三个方面阐述了全球和中国HIV-1基因亚型和新型重组型病毒演变的现状、特点和趋势, 以及正在发展的可助力于精准干预艾滋病流行的分子传播网络的最新研究进展, 包括全球HIV-1亚型多样性和分布地域性、我国HIV-1亚型此消彼长、新型重组病毒频繁发生和HIV分子传播网络研究进入实践阶段。本文明确指出了HIV-1亚型和重组病毒多样性是阻碍遏制HIV流行的关键因素, 并强调了基于分子簇的精准干预在助力控制艾滋病流行中的重要意义。本文将为从事艾滋病防治事业的公共卫生专业人员、医生以及实验室工作人员提供较新的信息, 供读者进一步学习和参考。

关键词: HIV/AIDS, 分子流行病学, 基因亚型, 重组病毒, 分子传播网络

Abstract: This article reviews the progress in global HIV-1 molecular epidemiology research and practice in recent years. The current diversity, characteristics and trends of the evolution of HIV-1 subtypes, as well as the emerging recombinant viruses in the world and China are described. The novel national strategy of molecular transmission network that can help to accurately intervene the AIDS epidemics is emphasized. This article will provides relatively new literatures for public health professionals, medical doctors and laboratory workers engaged in AIDS prevention and control. A large number of literatures and conference news cited in this article can be used for further reference by readers.

Key words: HIV/AIDS, Molecular epidemiology, Genetic subtype, Recombinant virus, Molecular transmission network

钟平. HIV分子流行病学研究和实践进展[J]. 新发传染病电子杂志, 2019, 4(3): 137-144.

ZHONG Ping. Progress in research and practice of molecular epidemiology of HIV-1[J]. Electronic Journal of Emerging Infectious Diseases, 2019, 4(3): 137-144.

参考文献

[1] WHO.Ten threats to global health in 2019[EB/OL].https://www.who.int/zh/emergencies/ten-threats-to-global-health-in-2019.
[2] UNAIDS. Global AIDS update 2018[M]. Geneva: UNAIDS, 2018: 12-18.
[3] 中国国家卫生健康委员会疾控局.2018年11月23日国务院新闻办发布会[EB/OL].http://www.chinadevelopmentbrief.org.cn/news-22265.html.
[4] 汪宁, 钟平. 中国HIV分子流行病学30年[J]. 中华流行病学杂志, 2015, 36(6):541-546.
[5] 钟平. 分子流行病学能否帮助将HIV的传播扼杀在萌芽状[J].中国热带医学, 2016, 16(11):1052-1056.
[6] HIV Databases Home.HIV Circulating Recombinant Forms (CRFs)[EB/OL].https://www.hiv.lanl.gov/content/sequence/HIV/CRFs/CRFs.html.
[7] Robertson DL, Anderson JP, Bradac JA, et al.HIV-1 nomenclature proposal[J]. Science, 2000, 288(5463):55-56.
[8] Hemelaar J, Elangovan R, Yun J.Global and regional molecular epidemiology of HIV-1, 1990-2015: systematic review, global survey, and trend analysis[J]. Lancet Infect Dis, 2019, 19(2):143-155.
[9] Kiwanuka N, Laeyendecker O, Quinn TC, et al.HIV-1 subtypes and differences in heterosexual HIV transmission among HIV-discordant couples in Rakai, Uganda[J]. AIDS, 2009, 23(18): 2479-2484.
[10] Bekker LG, Moodie Z, Grunenberg N, et al.Subtype C ALVAC-HIV and bivalent subtype C gp120/MF59 HIV-1 vaccine in low-risk, HIV-uninfected, South African adults: a phase 1/2 trial[J]. Lancet HIV, 2018, 5(7):e366-e378.
[11] Phanuphak N, Lo YR, Shao Y, et al.HIV Epidemic in Asia: Implications for HIV Vaccine and Other Prevention Trials[J]. AIDS Res Hum Retroviruses, 2015, 31(11):1060-1076.
[12] Liu Q, Li Y, Luo Z et al. HIV-1 vaccines based on replication-competent Tiantan vaccinia protected Chinese rhesus macaques from simian HIV infection[J]. AIDS, 2015, 29(6):649-658.
[13] Jin S, Ji Y, Wang Q, et al.Spatiotemporal hierarchy in antibody recognition against transmitted HIV-1 envelopeglycoprotein during natural infection[J].Retrovirology, 2016, 13(12):1-15.
[14] Paraskevis D, Hatzakis. Global molecular epidemiology of HIV-1:the chameleon chanllenge[J]. Lancet Infect Dis, 2019, 19(2):114-115.
[15] He X, Xing H, Ruan Y, et al.A comprehensive mapping of HIV-1 genotypes in various risk groups and regions across China based on a nationwide molecular epidemiologic survey[J]. PLoS One, 2012, 7(10):e47289.
[16] Han X, An M, Zhang W, et al.Genome Sequences of a Novel HIV-1 Circulating Recombinant Form, CRF55_01B, Identified in China[J]. Genome Announc, 2013, 1(1):1-4.
[17] Zhao J, Cai W, Zheng C, et al.Origin and outbreak of HIV-1 CRF55¬¬_01B among MSM in Shenzhen, China[J]. J Acquir Immune Defic Syndr, 2014, 66(3):e65-e67.
[18] Han X, Takebe Y, Zhang W, et al.A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact[J]. Sci Rep, 2015, 5:18147.
[18] Li J, Feng Y, Shen Z, et al.HIV-1 Transmission Among Recently Infected Individuals in Southwest China are Predominantly Derived from Circulating Local Strains[J]. Sci Rep, 2018, 8(1):12831.
[20] Zhou P, Yu G, Kuang Yi, et al.Rapid and complicated HIV genotype expansion among high-risk groups in Guangdong Province, China[J]. BMC Infect Dis, 2019, 19:185.
[21] Han X, An M, Zhang W, et al.Genome Sequences of a Novel HIV-1 Circulating Recombinant Form (CRF59_01B) Identified among Men Who Have Sex with Men in Northeastern China[J].Genome Announc, 2013, 1(3): e00315-13.
[22] Zhang W, Han X, An M, et al.Identification and characterization of a novel HIV-1 circulating recombinant form (CRF59_01B) identified among men-who-have-sex-with-men in China[J]. PLoS One, 2014, 9(6):e99693.
[23] Wu J, Meng Z, Xu J, et al.New emerging recombinant HIV-1 strains and close transmission linkage of HIV-1 strains in the Chinese MSM population indicate a new epidemic risk[J]. PLoS One, 2013, 8(1):e54322.
[24] Su L, Wei D, Yang H, et al.Identification of a Novel HIV-1 Circulating Recombinant Form (CRF85_BC) in Sichuan, China[J]. AIDS Res Hum Retroviruses, 2016, 32(9):895-859.
[25] Li Y, Han Y, Xie J, at al. CRF01_AE subtype is associated with X4 tropism and fast HIV progression in Chinese patients infected through sexual transmission[J]. AIDS, 2014, 28(4):521-530.
[26] Li X, Xue Y, Zhou L, et al.Evidence that HIV-1 CRF01_AE is associated with low CD4+T cell count and CXCR4 co-receptor usage in recently infected young men who have sex with men (MSM) in Shanghai, China[J]. PLoS One, 2014, 9(2):e89462.
[27] Cui H, Geng W, Sun H, et al.Rapid CD4+ T-cell decline is associated with coreceptor switch among MSM primarily infected with HIV-1 CRF01_AE in Northeast China[J]. AIDS, 2019, 33(1):13-22.
[28] Song H, Ou W, Feng Y, et al.Disparate impact on CD4 T cell count by two distinct HIV-1 phylogenetic clusters from the same clade[J]. Proc Natl Acad Sci USA, 2019, 116(1):239-244.
[29] Cohen J. MOLECULAR EPIDEMIOLOGY.HIV family trees reveal viral spread[J]. Science, 2015, 348(6240):1188-1189.
[30] Aldous JL, Pond SK, Poon A, et al.Characterizing HIV transmission networks across the United States[J]. Clin Infect Dis, 2012, 55(8):1135-1143.
[31] Little SJ, Kosakovsky SL, Anderson CM, et al.Using HIV networks to inform real time prevention interventions[J].PLoS One, 2014, 9(6):e98443.
[32] Wertheim JO, Kosakovsky SL, Forgione LA, et al.Social and Genetic Networks of HIV-1 Transmission in New York City[J]. PLoS Pathog, 2017, 13(1):e1006000.
[33] Pasquale DK, Doherty IA, Sampson LA, et al.Leveraging Phylogenetics to Understand HIV Transmission and Partner Notification Networks[J]. J AIDS, 2018, 78(4):367-375.
[34] Morgan E, Skaathun B, Schneider JA. Sexual, Social, Genetic Network Overlap: A Socio-Molecular Approach Toward Public Health Intervention of HIV[J]. Am J Public Health, 2018, 108(11):1528-1534.
[35] Investigating and Responding HIV Transmission Clusters. March2017, Version 1;Detecting and responding to HIV Transmission Cluster: A Guide for Health Departments, June 2018, Version 2.0.[EB/OL]. https://www.cdc.gov/hiv/programresources/guidance/cluster-outbreak/index.html.
[36] Oster AM, France AM, Mermin J.Molecular Epidemiology and the Transformation of HIV Prevention[J]. JAMA, 2018, 319(16):1657-1658.
[37] 黎裕雪云, 冯毅, 阮玉华, 等. HIV分子传播网络研究进展[J].中国艾滋病性病, 2016, 22(11):940-942.
[38] 李伟, 钟平, 吴波, 等.HIV-1分子传播网络分析及应用[J].中国艾滋病性病, 2018, 24(12):1258-1261.
[39] Liu AY, Buchbinder SP.CROI 2017: HIV Epidemic Trends and Advances in Prevention[J]. Top Antivir Med, 2017, 25(2):35-50.
[40] Campbell EM, Jia H, Shankar A, et al.Detailed Transmission Network Analysis of a Large Opiate-Driven Outbreak of HIV Infection in the United States[J]. J Infect Dis, 2017, 216(9):1053-1062.
[41] An M, Han X, Xu J, et al.Reconstituting the epidemic history of HIV strain CRF01_AE among men who have sex with men (MSM) in Liaoning, northeastern China: implications for the expanding epidemic among MSM in China[J]. J Virol, 2012, 86(22):12402-12406.
[42] Feng Y, He X, Hsi JH, et al.The rapidly expanding CRF01_AE epidemic in China is driven by multiple lineages of HIV-1 viruses introduced in the 1990s[J]. AIDS, 2013, 27(11):1793-1802.
[43] Wang X, He X, Zhong P, et al.Phylodynamics of major CRF01_AE epidemic clusters circulating in mainland of China[J]. Sci Rep, 2017, 7(1):6330.
[44] Li X, Xue Y, Lin Y, et al.Evolutionary Dynamics and Complicated Genetic Transmission Network Patterns of HIV-1 CRF01_AE among MSM in Shanghai, China[J]. Sci Rep, 2016, 6:34729.
[45] Li X, Zhu K, Xue Y, et al.Multiple introductions and onward transmission of HIV-1 subtype B strains in Shanghai, China[J]. J Infect, 2017, 75(2):160-168.
[46] Li X, Gao R, Zhu K, et al.Genetic transmission networks reveal the transmission patterns of HIV-1 CRF01_AE in China[J]. Sex Transm Infect, 2018, 94(2):111-116.
[47] Oster AM, France AM, Panneer N, et al.Identifying Clusters of Recent and Rapid HIV Transmission Through Analysis of Molecular Surveillance Data[J]. J AIDS, 2018, 79(5):543-550.
[48] Han Xiaoxu, Chaillon Antoine, Zhong Ping, et al. Dynamics of the CRF01_AE Epidemics in the Cities of Shanghai, Shenzhen and Shenyang[J]. CORI2018, Abstract No.945.
[49] Poon AF, Gustafson R, Daly P.Near real time monitoring of HIV transmission hotspots from routine HIV genotyping: an implementation case study[J]. Lancet HIV, 2016, 2(5):e231-238.
[50] Wang X, Wu Y, Mao L, et al.Targeting HIV Prevention Based on Molecular Epidemiology Among Deeply Sampled Subnetworks of Men Who Have Sex With Men[J]. Clin Infect Dis, 2015, 61(9):1462-1468.
[51] 林怡, 薛以乐, 王绪琴, 等.上海市男男性行为者中感染HIV-1的CRF01_AE毒株传播簇分析[J].中国艾滋病性病, 2016, 22(3):165-169.
[52] 赵帅, 冯毅, 辛若雷, 等.应用分子传播网络研究北京男男性行为者HIV-1毒株的传播特征[J].中国艾滋病性病, 2018, 24(3):250-255.
[53] 罗明宇, 潘晓虹, 范钦, 等.浙江省嘉兴市2017年较大HIV分子传播簇病例流行病学特征分析[J].中华流行病学杂志, 2019, 40(2):202-206.
[54] Su L, Liang S, Hou X, et al.Impact of worker emigration on HIV epidemics in labour export areas: a molecular epidemiology investigation in Guangyuan, China[J]. Sci Rep, 2018, 8(1):16046.
[55] Chen M, Ma Y, Chen H, et al.HIV-1 genetic transmission networks among men who have sex with men in Kunming, China[J]. PLoS One, 2018, 13(4):e0196548.
[56] Fauci AS, Redfield RR, Sigounas G, et al. Ending the HIV Epidemic:A Plan for the United States[J]. JAMA, 2019 Feb 7. Doi:10.1001/jama.2019. 1343.