右美托咪定对结核病大鼠的T淋巴细胞、NK细胞和γ-干扰素表达和肺损伤的影响

doi:10.19871/j.cnki.xfcrbzz.2021.02.004

新发传染病电子杂志 ›› 2021, Vol. 6 ›› Issue (2): 92-96.doi: 10.19871/j.cnki.xfcrbzz.2021.02.004

右美托咪定对结核病大鼠的T淋巴细胞、NK细胞和γ-干扰素表达和肺损伤的影响

唐怡敏¹, 洪凤珠¹, 王进¹, 刘民强², 韩亚坤², 余德慧², 邓永聪¹

1.深圳市第三人民医院肺科门诊,广东深圳 518112;
2.深圳市第三人民医院麻醉科,广东深圳 518112

收稿日期:2021-02-23 出版日期:2021-05-31 发布日期:2021-06-24
通讯作者: 邓永聪,E-mail：531070121@qq.com
基金资助:
广东省高水平临床重点专科（深圳市配套建设经费）资助（SZGSP010）

Influences of pretreating with dexmedetomidine on the expression of T lymphocyte,natural killer cells, interferon-gamma, and lung injury in tuberculosis rats after operation

Tang Yimin¹, Hong Fengzhu¹, Wang Jin¹, Liu Minqiang², Han Yakun², Yu Dehui², Deng Yongcong¹

1. Department of Pulmonary Clinica1, the Third People's Hospital of Shenzhen, Guangdong Shenzhen 518112, China;
2. Department of Anesthesiology, the Third People's Hospital of Shenzhen, Guangdong Shenzhen 518112, China

Received:2021-02-23 Online:2021-05-31 Published:2021-06-24

摘要/Abstract

摘要： 目的观察右美托咪定（dexmedetomidine,DEX）预处理对结核病（tuberculosis,TB）大鼠CD3⁺T淋巴细胞、CD4⁺T淋巴细胞、CD8⁺T淋巴细胞、自然杀伤细胞（natural killer cell,NK细胞）、γ-干扰素（interferon gamma,IFN-γ）表达及肺损伤的影响,为TB患者手术期间免疫保护提供参考。方法取SD大鼠30只,随机分为非结核对照组（C组）、结核+生理盐水对照组（T组）、结核+DEX 5μg/kg组（D1组）、结核+DEX 10μg/kg组（D2组）和结核+DEX 15μg/kg组（D3组）,每组6只。采用流式细胞仪检测麻醉前30min（T0）,手术后1h（T1）、4h（T2）及8h（T3）眼眶静脉血CD3⁺T淋巴细胞、CD4⁺T淋巴细胞、CD8⁺T淋巴细胞、CD4⁺T淋巴细胞/CD8⁺T淋巴细胞比值、NK细胞及IFN-γ表达水平的变化;术后12h处死大鼠,观察肺组织的病理学改变。结果与T0比较,各组术后T淋巴细胞亚群、NK细胞及IFN-γ均出现抑制,但同一时点各组间T淋巴细胞亚群、NK细胞及IFN-γ的变化差异无统计学意义（P>0.05）,且TB大鼠术后肺部炎症反应程度相似。结论 TB大鼠围术期采用DEX预处理无明显免疫保护及肺保护作用,该药在TB患者中使用需谨慎。

关键词: 右美托咪定, 结核病, T淋巴细胞, 自然杀伤细胞, γ-干扰素

Abstract: Objective To observe the influences of pretreating with dexmedetomidine (DEX) on the expression of CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD8⁺T lymphocyte, natural killer cell (NK cell), interferon-gamma (IFN-γ), and lung injury in tuberculosis (TB) rats after operation, to provide reference for immune protection of TB patients during operation. Methods Thirty Sprague Dawley (SD) rats were enrolled in this study, and were randomly divided into 5 groups, including non-tuberculosis control group (group C), tuberculosis + saline control group (group T), tuberculosis + DEX 5μg/kg group (group D1), tuberculosis + DEX 10μg/kg group (group D2) and tuberculosis + DEX 15μg/kg group (group D3) with 6 cases each. Blood samples were taken from the orbital venous at 30 min before anesthesia (T0),1 hour (T1), 4 hours (T2) and 8 hours (T3) after operation, and CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD8⁺T lymphocyte, CD4⁺/CD8⁺ratio, NK cell, and interferon gamma (IFN-γ) expression were calculated with flow cytometer. The lung pathologies were examined 12 hours after operation. Results Compared with T0, the T lymphocyte subsets, NK cell and IFN-γ in each group were inhibited. But there was no significant difference in the changes of T lymphocyte subsets, NK cell and IFN-γ in each group (P>0.05), and the degree of pulmonary inflammation was similar at the same time point in each group. Conclusions There was no obvious immune protection and lung protection effect on TB rats pretreated with DEX during perioperative period. It has no lung protection effect on TB rats, thus it should be used cautiously in TB patients.

Key words: Dexmedetomidine, Tuberculosis, T lymphocyte, Natural killer cells, Interferon-gamma

唐怡敏, 洪凤珠, 王进, 刘民强, 韩亚坤, 余德慧, 邓永聪. 右美托咪定对结核病大鼠的T淋巴细胞、NK细胞和γ-干扰素表达和肺损伤的影响[J]. 新发传染病电子杂志, 2021, 6(2): 92-96.

Tang Yimin, Hong Fengzhu, Wang Jin, Liu Minqiang, Han Yakun, Yu Dehui, Deng Yongcong. Influences of pretreating with dexmedetomidine on the expression of T lymphocyte,natural killer cells, interferon-gamma, and lung injury in tuberculosis rats after operation[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(2): 92-96.

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右美托咪定对结核病大鼠的T淋巴细胞、NK细胞和γ-干扰素表达和肺损伤的影响

Influences of pretreating with dexmedetomidine on the expression of T lymphocyte,natural killer cells, interferon-gamma, and lung injury in tuberculosis rats after operation

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