锌指抗病毒蛋白在肠道病毒71型感染小鼠中的表达及其意义

doi:10.19871/j.cnki.xfcrbzz.2022.01.002

新发传染病电子杂志 ›› 2022, Vol. 7 ›› Issue (1): 6-11.doi: 10.19871/j.cnki.xfcrbzz.2022.01.002

锌指抗病毒蛋白在肠道病毒71型感染小鼠中的表达及其意义

谢丽¹, 王峰¹, 钟育权², 杨慧¹

1.深圳市慢性病防治中心中心实验室,广东深圳 518020;
2.广东医科大学,广东东莞 523109

收稿日期:2021-12-20 出版日期:2022-02-28 发布日期:2022-07-07
通讯作者: 杨慧,Email: yh2009cn@qq.com
基金资助:
深圳市卫生系列科研项目资助（SZBC2018009）

Expression and significance of zinc-finger antiviral protein in enterovirus 71 infected mice

Xie Li¹, Wang Feng¹, Zhong Yuquan², Yang Hui¹

1. Department of Central Lab, Shenzhen Center For Chronic Disease Control,Guangdong Shenzhen 518020, China;
2. Guangdong Medical University, Guangdong Dongguan 523109, China

Received:2021-12-20 Online:2022-02-28 Published:2022-07-07

摘要/Abstract

摘要： 目的探索锌指抗病毒蛋白（ZAP）在肠道病毒71型（EV71）感染小鼠中的表达水平及其调节作用,为EV71抗病毒治疗提供数据基础。方法首先将C57BL/6小鼠随机分为4组,分别为空白对照组（Control组）、感染组（EV71组）、感染+ZAP质粒组（ZAP组）、感染+空质粒组（Mock组）。后3组腹腔注射EV71建立感染小鼠模型,ZAP组小鼠通过眼眶后静脉丛注射质粒构建ZAP过表达小鼠模型。采用实时荧光定量聚合酶链反应、苏木精-伊红染色和蛋白质印迹法等方法,检测小鼠不同组织病毒载量、各组织病变情况、ZAP和VP1表达水平。结果 EV71感染后小鼠组织中,ZAP的表达显著增加;EV71感染后,ZAP过表达小鼠病理损伤减轻,组织中病毒载量降低,与野生型小鼠相比表现出更轻的症状。结论 ZAP在抗EV71感染的免疫过程中扮演重要角色,提示ZAP过表达可以提高小鼠对EV71病毒的敏感性,并提高对病毒性炎症反应的抵抗力。这一发现为ZAP在病毒诱导的炎症性疾病中的作用提供了新的思路。

关键词: 锌指抗病毒蛋白, 肠道病毒71型, 小鼠模型, 病毒和宿主的相互作用

Abstract: Objective To explore the expression level of host protein ZAP in enterovirus 71 (EV71) infected mice and its regulatory effect, and to provide data basis for antiviral treatment of EV71. Method The C57BL/6 mice were randomly divided into 4 groups, blank control group (Control group), infection group (EV71 group), infection + ZAP plasmid group (ZAP group) and infection + empty plasmid group (Mock group). In the latter three groups, EV71 was injected intraperitoneally to establish the infection model, and the mice in the plasmid injection group were injected with plasmids into the retro-orbital venous plexus to construct a ZAP overexpression mouse model. Hematoxylin-eosin (HE) staining, Western blot and quantitative real time polymerase chain reaction (Q-PCR) were used to detect mice. Viral load, pathological changes and expression levels of ZAP and VP1 in different tissues were detected. Conclusion ZAP plays an important role in the immune response to EV71 infection, suggesting that ZAP overexpression can improve the sensitivity of mice to EV71 virus and the resistance to viral inflammation. This finding provides new insights into the role of ZAP in virus-induced inflammatory disease.

Key words: Zinc-finger antiviral protein, Enterovirus 71, Mouse model, Interaction between virus and host

谢丽, 王峰, 钟育权, 杨慧. 锌指抗病毒蛋白在肠道病毒71型感染小鼠中的表达及其意义[J]. 新发传染病电子杂志, 2022, 7(1): 6-11.

Xie Li, Wang Feng, Zhong Yuquan, Yang Hui. Expression and significance of zinc-finger antiviral protein in enterovirus 71 infected mice[J]. Electronic Journal of Emerging Infectious Diseases, 2022, 7(1): 6-11.

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锌指抗病毒蛋白在肠道病毒71型感染小鼠中的表达及其意义

Expression and significance of zinc-finger antiviral protein in enterovirus 71 infected mice

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