用于肺结核诊断的痰涂片自动镜检系统双中心临床应用 评价

doi:10.19871/j.cnki.xfcrbzz.2022.04.013

新发传染病电子杂志 ›› 2022, Vol. 7 ›› Issue (4): 57-60.doi: 10.19871/j.cnki.xfcrbzz.2022.04.013

用于肺结核诊断的痰涂片自动镜检系统双中心临床应用评价

赵豆豆¹, 何慧珊^2#, 夏丽³, 郑秋婷⁴, 钱令军³, 洪坤磊³, 刘远明³, 陶丽丽⁵, 郭琳³

1.新疆医科大学第八附属医院检验科,乌鲁木齐 830013;
2.深圳市慢性病防治中心病原检验科,广东深圳 518020;
3.深圳市智影医疗科技有限公司,广东深圳 518109;
4.深圳市慢性病防治中心结核病影像科,广州深圳 518020;
5.北京大学深圳医院病理科,广东深圳 518036

收稿日期:2021-08-30 发布日期:2023-02-20
通讯作者: 郭琳,Email：guolin913@outlook.com;陶丽丽,Email：651055473@qq.com
作者简介:#：并列第一作者
基金资助:
1.国家重点研发计划项目（2019YFE0121400）; 2.深圳市科技计划项目（KQTD2017033110081833; JSGG20201102162802008）; 3.深圳市基础研究项目（JCYJ20190813153413160）

Dual-center clinical evaluation of sputum smear automatic microscopy system for detecting tuberculosis

Zhao Doudou¹, He Huishan², Xia Li³, Zheng Qiuting⁴, Qian Lingjun³, Hong Kunlei³, Lure Fleming Yuan-ming³, Tao Lili⁴, Guo Lin³

1. Department of Laboratory Medicine, the Eighth Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi, 830013, China;
2. Department of Pathogenic Laboratory, Shenzhen Chronic Disease Control Center Shenzhen, Guangdong Shenzhen 518020, China;
3. Shenzhen Zhiying Medical Imaging Co., Ltd, Guangdong Shenzhen 518109, China;
4. Department of Medical Imaging, Shenzhen Chronic Disease Control Center Shenzhen, Guangdong Shenzhen 518020, China;
5. Department of Pathology, Peking University Shenzhen Hospital, Guangdong Shenzhen 518036, China

Received:2021-08-30 Published:2023-02-20

摘要/Abstract

摘要： 目的评价痰涂片自动镜检系统（ASSMS）在结核分枝杆菌筛检上的效果和价值,探究该系统在临床使用过程中的应用方式。方法回顾性地收集了新疆医科大学第八附属医院（中心Ⅰ）的474份痰标本以及深圳市慢性病防治中心（中心Ⅱ）的451份痰标本制作痰涂片,然后分别采用人工阅片方式和自动镜检系统阅片方式进行痰检,最后评估ASSMS阅片在临床上的应用价值。结果 2个中心的925例样本中,ASSMS阅片的检出率高于人工阅片（中心Ⅰ：P<0.05,中心Ⅱ：P>0.05）。中心Ⅰ的实验结果显示,人工阅片与ASSMS阅片的准确率、灵敏度和特异度分别为91.8 %和90.5%、74.4%和86.7%、97.7%和91.8%,ASSMS阅片可以检出31份被医生漏诊的痰标本（25.8%）。中心Ⅱ的实验结果显示,人工阅片与ASSMS阅片的准确率、灵敏度和特异度分别为90.7%和85.8%、52.3%和68.2%、94.8%和87.7%,ASSMS阅片可以检出21份被医生漏诊的痰标本（47.7%）。结论采用ASSMS阅片的方式辅助人工阅片可以提高阳性检出率,减少医生漏诊病例。

关键词: 痰涂片, 自动显微镜, 结核分枝杆菌, 对比研究

Abstract: Objective To evaluate the mycobacterium tuberculosis detection performance of ASSMS and compare it with that of the traditional manual way. Method 474 and 451 sputum specimens were retrospectively collected from Eighth Affiliated Hospital of Xinjiang Medical University (Center Ⅰ) and Shenzhen Chronic Disease Control Center Shenzhen (Center Ⅱ), respectively, and mycobacterium tuberculosis tests were separately conducted by doctors and ASSMS after specimens were made into sputum smears. Results were finally compared and analyzed. Result The detection rate of ASSMS was higher than manual detection on two datasets of 925 cases (Center Ⅰ: P<0.05,Center Ⅱ: P>0.05). The results of center Ⅰshowed that the accuracy, sensitivity and specificity of manual and automatic detection were 91.8% vs 90.5%, 74.4% vs 86.7% and 97.7% vs 91.8%, and the ASSMS finally identified 31 sputum samples (25.8%)that was missed by doctors. While the results from center Ⅱ showed that the accuracy, sensitivity and specificity of manual and automatic detection were 90.7% vs 85.8%, 52.3% vs 68.2% and 94.8% vs 87.7%, and the ASSMS identified 21 sputum samples (47.7%) doctors missed. Conclusion Automatic detection may be functioned as an effective assistant to help doctors reduce missed diagnosis rate of tuberculosis.

Key words: Tuberculosis, Sputum smear, Automated microscopy, Mycobacterium tuberculosis, Comparative study

赵豆豆, 何慧珊, 夏丽, 郑秋婷, 钱令军, 洪坤磊, 刘远明, 陶丽丽, 郭琳. 用于肺结核诊断的痰涂片自动镜检系统双中心临床应用评价[J]. 新发传染病电子杂志, 2022, 7(4): 57-60.

Zhao Doudou, He Huishan, Xia Li, Zheng Qiuting, Qian Lingjun, Hong Kunlei, Lure Fleming Yuan-ming, Tao Lili, Guo Lin. Dual-center clinical evaluation of sputum smear automatic microscopy system for detecting tuberculosis[J]. Electronic Journal of Emerging Infectious Diseases, 2022, 7(4): 57-60.

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用于肺结核诊断的痰涂片自动镜检系统双中心临床应用评价

Dual-center clinical evaluation of sputum smear automatic microscopy system for detecting tuberculosis

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用于肺结核诊断的痰涂片自动镜检系统双中心临床应用 评价

Dual-center clinical evaluation of sputum smear automatic microscopy system for detecting tuberculosis

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用于肺结核诊断的痰涂片自动镜检系统双中心临床应用评价