电喷雾萃取电离质谱技术在流行病学中的应用

doi:10.19871/j.cnki.xfcrbzz.2022.04.020

摘要/Abstract

摘要： 电喷雾萃取电离质谱（EESI-MS）结合了电喷雾电离（ESI）技术和液液萃取分离技术的优点,可对不同复杂样品进行实时在线的直接质谱分析,其具有基体耐受性强、稳定性好、灵敏度高、适用面广的特点,现已逐步成为生物活体分析、原位在线分析和远距离分析的一种有效质谱分析方法。对于液体、气体等样品,EESI-MS可以直接进行有效的质谱分析。对于胶体及活体植物等样品,EESI-MS技术可以采用中性气体解吸采样,可实现无化学污染、无生物应激、没有明显损伤的温和质谱分析,对动植物生理或病理状态没有明显干扰。更重要的是,采用改进后的内部萃取电喷雾电离质谱（iEESI-MS）技术,可在无需匀浆、萃取等样品预处理的条件下,直接实现组织样品内部痕量物质的定性定量分析。由此可见EESI-MS技术具有功能强大、适用面广等特点。近年来,EESI-MS在流行病学中的应用较为广泛。流行病学的研究范畴较广,其研究对象除了传染病和慢性病外,还包括对环境、劳动、食品等领域中影响人体健康的有害物质的分析。本文主要评述EESI-MS技术在流行病学中的应用,阐述其在传染病和慢性病的分子诊断上方面具有的特殊优势,以促进其在相关领域的交流和发展。

关键词: 电喷雾萃取电离质谱, 流行病学, 传染病, 慢性病, 疾病诊断

Abstract: Extractive electrospray ionization mass spectrometry (EESI-MS) combines the advantages of electrospray ionization and liquid-liquid extraction. EESI-MS can be used for real-time mass spectrometry analysis of complex samples, featuring the characteristics of strong tolerance of biomatrices, good stability, high sensitivity, and wide applicability. It has become an effective mass spectrometry method for in vivo analysis, in-situ analysis, and long-distance analysis. For liquidand gas, EESI-MS can be directly carried out to obtain analytical data. For actual samples such as colloids, heterogeneous mixtures and living plant surface, EESI-MS technology can adopt neutral gas desorption sampling, which can realize mild mass spectrometry analysis without chemical pollution, biological stress, and obvious damage, and has no obvious interference to the physiological or pathological state of animals and plants. More importantly, the improved internal extraction electrospray ionization (iEESI) MS technology can directly and quantitatively achieve trace amounts of trace substances in tissue samples without pretreatment of homogenate and extraction. Therefore, EESI-MS technology has the characteristics of powerful function and wide application. In recent years, EESI-MS has been widely used in epidemiology, which includes the analysis of infectious diseases and chronic diseases, the detection of harmful substances affecting human health in the fields of environment, labor and food. EESI-MS has special advantages in the molecular diagnosis of infectious diseases and chronic diseases. Due to the limitation of length and theme, this paper mainly reviews the application of EESI-MS technology in epidemiology to promote the exchange and development of related fields.

Key words: Extractive electrospray ionization mass spectrometry, Epidemiology, Infectious diseases, Chronic disease, Diagnosis of disease

翟兴英, 龙资, 张兴磊, 朱腾高, 刘坤, 徐加泉. 电喷雾萃取电离质谱技术在流行病学中的应用[J]. 新发传染病电子杂志, 2022, 7(4): 86-90.

Zhai Xingying, Long Zi, Zhang Xinglei, Zhu Tenggao, Liu Kun, Xu Jiaquan. Application of extractive electrospray ionization mass spectrometry in epidemiology[J]. Electronic Journal of Emerging Infectious Diseases, 2022, 7(4): 86-90.

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