东莞市HIV-1流行株pol基因序列特征分析

doi:10.19871/j.cnki.xfcrbzz.2023.06.002

新发传染病电子杂志 ›› 2023, Vol. 8 ›› Issue (6): 9-13.doi: 10.19871/j.cnki.xfcrbzz.2023.06.002

东莞市HIV-1流行株pol基因序列特征分析

何松美¹, 梁金虎², 钟庆杨³, 曹焕焕¹, 尹菊侦², 王福祥²

1.东莞市人民医院感染科,广东东莞 523058;
2. 深圳市第三人民医院(南方科技大学附属第二医院)感染中心,广东深圳 518112;
3.东莞市第九人民医院感染科,广东东莞 523018

收稿日期:2023-10-15 发布日期:2024-01-23
通讯作者: 王福祥,Email:13927486077@163.com
基金资助:
1.国家自然科学基金面上项目（81971915,82272318）; 2.深圳市科技研发基金（JCYJ20200109144203815）; 3.广东省高水平临床重点专科（深圳市配套建设经费）资助（SZGSPOI1); 4.基于干细胞技术的重大传染病临床救治方案研究（2022YFC2304403,2022YFC2304404）

Sequence analysis of pol gene of HIV-1 strain in Dongguan

He Songmei¹, Liang Jinhu², Zhong Qingyang³, Cao Huanhuan¹, Yin Juzhen², Wang Fuxiang²

1. Department of Infection, Dongguan People's Hospital, Guangdong Dongguan 523058, China;
2. Department of Infectious Diseases, The Third People's Hospital of Shenzhen, The Second Affiliated Hospital of Southern University of Science and Technology, Guangdong Shenzhen 518112, China;
3. Department of Infection, Dongguan Ninth People's Hospital, Guangdong Dongguan 523018, China

Received:2023-10-15 Published:2024-01-23

摘要/Abstract

摘要： 目的了解东莞市HIV-1感染者的病毒流行特征及亚型的分布情况。方法收集2019年1月至2021年1月东莞市未接受抗病毒治疗的HIV-1感染者284例,收取其基本人口学信息,采集血液样本并提取病毒RNA,采用巢式聚合酶链反应扩增HIV-1 pol段基因,应用LaserGene、HIV BLAST、MEGA 6.0等生物学软件进行基因序列分析。结果本研究纳入284例样本,最终成功获得269条HIV-1 pol基因序列。269份样本中,86.2%为男性,61.3%年龄<40岁,52.4%为未婚,异性性传播占比49.4%,同性性传播占比47.2%。269条pol基因序列中,共发现8种基因亚型,其中主要亚型为CRF07_BC（48.7%）、CRF01_AE（21.9%）和CRF55_01B（14.1%）。各个亚型感染者在性别和婚姻状况的构成上差异有统计学意义（P<0.05）,在年龄和感染途径的构成上差异无统计学意义。结论东莞市HIV-1亚型分布复杂多样,以CRF07_BC、CRF01_AE和CRF55_01B为主,且在不同性别、不同婚姻状况的HIV感染者中存在显著差异。

关键词: 人类免疫缺陷病毒Ⅰ型, 序列分析, 亚型分布

Abstract: Objective To investigate the epidemiological characteristics of human immunodeficiency virus type 1 (HIV-1) and the subtypes distribution among HIV-1-infected individuals in Dongguan. Method From January 2019 to January 2021, 284 HIV-1 infected patients who had received antiviral treatment in Dongguan were selected, and was collected their epidemiological information. The viral RNA of HIV-1 was extracted from plasma. The pol gene was amplified by nested polymerase chain reaction. and gene sequence analysis was performed using biological software such as LaserGene, HIV BLAST, and MEGA 6.0. Result A total of 284 samples were collected in this study and finally successfully obtained 269 HIV-1 pol gene sequences. Among the 269 samples, 86.2% (232/269) were male, 61.3% (165/269) were younger than 40 years old, 52.4% (141/269) were unmarried, and 49.4% (133/269) were heterosexual and 47.2% (127/269) were homosexual. Of the 269 pol gene sequences, eight genetic subtypes were identified: 131 (48.7%) CRF07_BC, 59 (21.9%) CRF01_AE, 38 (14.1%) CRF55_01B, 19 (7.1%) subtype B, 12 (4.5%) CRF08_BC, and 10 (3.7%) other subtypes. Among 133 patients with heterosexual transmission, the predominant subtypes were 59 (44.4%) CRF07_BC, 32 (24.1%) CRF01_AE, and 15 (11.3%) CRF55_01B, and among 127 patients with homosexual transmission, the predominant subtypes were 65 (51.2%) CRF07_BC, 26 (20.5%) CRF01_AE, and 23 (18.1%) CRF55_01B. Differences in gender and marital status composition among individuals infected with different subtypes were statistically significant (P<0.05), while no statistically significant differences were observed in age and mode of infection composition. Conclusion The distribution of HIV-1 subtypes in Dongguan is complex and diverse, with CRF07_BC, CRF01_AE, and CRF55_01B being predominant. Furthermore, significant differences exist among HIV-infected individuals of different genders and marital statuses.

Key words: Human immunodeficiency virus typeⅠ, Sequence analysis, Subtype distribution

中图分类号:

R512.91

何松美, 梁金虎, 钟庆杨, 曹焕焕, 尹菊侦, 王福祥. 东莞市HIV-1流行株pol基因序列特征分析[J]. 新发传染病电子杂志, 2023, 8(6): 9-13.

He Songmei, Liang Jinhu, Zhong Qingyang, Cao Huanhuan, Yin Juzhen, Wang Fuxiang. Sequence analysis of pol gene of HIV-1 strain in Dongguan[J]. Electronic Journal of Emerging Infectious Diseases, 2023, 8(6): 9-13.

参考文献

[1] SEPKOWITZ KA.Opportunistic Infections in Patients with and Patients without Acquired Immunodeficiency Syndrome[J]. Clin Infect Dis, 2002, 34(8): 1098-1107.
[2] FINZI D, HERMANKOVA M, PIERSON T, et al.Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy[J]. Science, 1997, 278(5341): 1295-1300.
[3] WONG JK, HEZAREH M, GUNTHARD HF, et al.Recovery of replication-competent HIV despite prolonged suppression of plasma viremia[J]. Science, 1997, 278(5341): 1291-1295.
[4] GAO D, ZOU Z, DONG B, et al.Secular trends in HIV/AIDS mortality in China from 1990 to 2016: gender disparities[J]. PLoS One, 2019, 14(7): e0219689.
[5] SONG H, GIORGI EE, GANUSOV VV, et al.Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection[J]. Nat Commun, 2018, 9(1): 1928.
[6] ZHOU PP, YU G, KUANG YQ, et al.Rapid and complicated HIV genotype expansion among high-risk groups in Guangdong Province, China[J]. BMC Infect Dis, 2019, 19(1): 185.
[7] 于鑫, 赵鹏宇, 岳超, 等. 哈尔滨市艾滋病患者抗病毒治疗耐药情况及影响因素分析[J]. 中华疾病控制杂志, 2016, 20(12): 1194-1198.
[8] BAIRD HA, GAO Y, GALETTO R, et al.Influence of sequence identity and unique breakpoints on the frequency of intersubtype HIV-1 recombination[J]. Retrovirology, 2006, 3(1): 91.
[9] 苏迎盈, 刘慧鑫, 吴静, 等. 中国HIV-1基因亚型分布及流行趋势[J]. 中华流行病学杂志, 2014, 10: 1164-1168.
[10] 杨普贤, 汤赵荣, 刘伟元, 等. 2002~2019年东莞市大岭山镇艾滋病疫情数据统计及流行病学特征分析[J]. 中国实用医药, 2021, 16(3): 134-136.
[11] 刘绍青, 覃晓丹, 徐永芳, 等. 2016—2021年南宁市≥50岁人群HIV/AIDS病例流行特征分析[J]. 医学动物防制, 2023, 39(11): 1077-1079,1084.
[12] 成慧, 刘登来, 刘家虹, 等. 江西省某县嫖客人群艾滋病流行病学分析[J]. 中国公共卫生, 2018, 34(9): 1295-1298.
[13] 陈献军, 陈允刚, 梁英芳, 等. 参与式健康教育方法在农村艾滋病疫情严重地区的应用[J]. 应用预防医学, 2017, 23(3): 234-236.
[14] 伦玉华, 罗皓, 方晓君, 等. 东莞市HIV-1流行株pol基因序列特征和亚型分析[J]. 中华疾病控制杂志, 2018, 22(12): 1297-1299.
[15] LI Y, HAN Y, XIE J, et al.CRF01_AE subtype is associated with X4 tropism and fast HIV progression in Chinese patients infected through sexual transmission[J]. AIDS, 2014, 28(4): 521-530.
[16] GE Z, FENG Y, LI K, et al.CRF01_AE and CRF01_AE Cluster 4 Are Associated With Poor Immune Recovery in Chinese Patients Under Combination Antiretroviral Therapy[J]. Clin Infect Dis, 2021, 72(10): 1799-1809.
[17] ZAI J, LIU H, LU Z, et al.Tracing the transmission dynamics of HIV-1 CRF55_01B[J]. Sci Rep, 2020, 10(1): 5098.
[18] 方晓君, 罗皓, 伦玉华, 等. 东莞市HIV-1主要毒株基因分型及其对耐药影响的研究[J]. 华南预防医学, 2020, 46(3): 227-230,234.
[19] 钟平. HIV 分子流行病学研究和实践进展[J/CD]. 新发传染病电子杂志, 2019, 4(3): 137-144.
[20] ZHANG D, ZHENG C, LI H, et al.Molecular surveillance of HIV-1 newly diagnosed infections in Shenzhen, China from 2011 to 2018[J]. J Infect, 2021, 83(1): 76-83.
[21] 邱钰婷, 李健健, 刘家法, 等. 云南省昆明市HIV-1流行株pol基因序列特征分析[J]. 中华疾病控制杂志, 2022, 26(3): 309-314.
[22] 洪亮, 陶静, 李佳洁, 等. 上海市虹口区2016 年新报告HIV-1 感染者基因亚型和耐药监测情况[J]. 上海预防医学, 2022, 34(9): 860-864,878.
[23] 宋潇, 李婷, 江云飞, 等. 黑龙江省588例新报告HIV-1感染者病毒基因特征及耐药分析[J]. 中国艾滋病性病, 2022, 28(7): 781-785.
[24] LI QH, WANG JY, LIU SY, et al.Young MSM changed temporal HIV-1 epidemic pattern in Heilongjiang Province, China[J]. Front Microbiol, 2022, 13: 1028383.
[25] 乔瑞娟, 张林才, 冯毅, 等. 甘肃省部分地区2020-2021年新报告HIV/AIDS患者治前耐药及分子传播网络特征分析[J]. 中国艾滋病性病, 2023, 29(08): 860-865.

东莞市HIV-1流行株pol基因序列特征分析

Sequence analysis of pol gene of HIV-1 strain in Dongguan

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