乳酸脱氢酶、腺苷脱氨酶、可溶性生长刺激表达基因2 蛋白联合检测对结核性胸腔积液的诊断价值

doi:10.19871/j.cnki.xfcrbzz.2024.02.009

摘要/Abstract

摘要： 目的探讨结核性胸腔积液（pleural effusion,TPE）患者乳酸脱氢酶（lactate dehydrogenase,LDH）、腺苷脱氨酶（adenosine deaminase,ADA）、可溶性生长刺激表达基因2蛋白（soluble growth stimulation expressed gene 2,sST2）的表达水平,并分析3项联合检测对TPE的诊断价值。方法选取2021年9月至2023年9月于西部战区总医院就诊的101例胸腔积液患者,依据是否存在结核分枝杆菌感染分为TPE组52例,对照组（非结核分枝杆菌感染）49例。收集一般资料,胸膜腔穿刺术收集患者胸腔积液,采用酶比色法检测LDH水平,波氏比色法检测ADA水平,酶联免疫吸附法（enzyme linked immunosorbent assay,ELISA）检测sST2水平;应用受试者操作特征曲线（receiver operating characteristic curve,ROC曲线）分析LDH、ADA、sST2对TPE的诊断价值;多因素Logistic回归分析TPE发生的影响因素。结果 TPE组胸腔积液LDH、ADA与sST2水平高于对照组（P<0.05）。ROC曲线结果显示LDH、ADA、sST2单项及三项联合预测胸腔积液患者发生TPE的AUC分别为0.865、0.867、0.880、0.954,三项联合诊断优于LDH、ADA、sST2单项诊断（Z=2.981、2.232、2.689,P<0.05）;多因素Logistic回归分析显示LDH、ADA、sST2均是TPE发生的影响因素（P<0.05）。结论结核性胸腔积液患者LDH、ADA、sST2水平升高,三项联合检测对TPE具有一定的诊断价值。

关键词: 结核性胸腔积液, 胸膜腔穿刺术, 乳酸脱氢酶, 腺苷脱氨酶, 可溶性生长刺激表达基因2蛋白

Abstract: Objective To investigate the expression levels of lactate dehydrogenase (LDH), adenosine deaminase (ADA), and soluble growth stimulation expressed gene 2 (sST2) in patients with tuberculous pleural effusion and to analyze the diagnostic value of the three combined for tuberculous pleural effusion (TPE). Method From September 2021 to September 2023, 101 patients with pleural effusion who visited Western Theater Command General Hospital were selected. They were grouped into a TPE group of 52 cases and a control group of 49 cases (non tuberculosis infection) based on the presence of tuberculosis infection. General information was collected, pleural effusion was collected from patients through thoracentesis, enzyme colorimetry was applied to detect LDH level, pois colorimetric method was applied to detect ADA level, enzyme linked immunosorbent assay (ELISA) was applied to detect the level of sST2; receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of pleural fluid LDH, ADA, and sST2 for TPE; multivariate Logistic regression was applied to analyze the influencing factors of TPE occurrence. Result LDH, ADA and sST2 levels in pleural fluid were higher in the TPE group than in the control group, and the difference was statistically significant (P<0.05). ROC curve results showed that the AUC of LDH, ADA and sST2 alone and in combination in predicting TPE in patients with pleural effusion was 0.865, 0.867, 0.880, and 0.954, respectively, the combined diagnosis of the three was superior to the individual diagnosis of LDH, ADA, and sST2 (Z=2.981, 2.232, 2.689, P<0.05). Multifactorial Logistic regression analysis showed that LDH, ADA, and sST2 were all influential factors in the development of TPE (P<0.05). Conclusion LDH, ADA, and sST2 levels are increased in patients with tuberculous pleural effusion, and the combination of the three tests has some diagnostic value in tuberculous pleural effusion.

Key words: Tuberculous pleural effusion, Lactate dehydrogenase, Adenosine deaminase, Growth stimulation expressed gene 2

中图分类号:

李黎, 程义, 孙巍. 乳酸脱氢酶、腺苷脱氨酶、可溶性生长刺激表达基因2 蛋白联合检测对结核性胸腔积液的诊断价值[J]. 新发传染病电子杂志, 2024, 9(2): 46-49.

Li Li, Cheng Yi, Sun Wei. Diagnostic value of combined detection of lactate dehydrogenase, adenosine deaminase, and soluble growth stimulation expressed gene 2 for tuberculous pleural effusion[J]. Electronic Journal of Emerging Infectious Diseases, 2024, 9(2): 46-49.

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