Abstract: Objective To investigate the clinical features of tuberculosis patients with pyrazinamide(PZA) resistance. Methods Twenty-two patients who were hospitalized in our hospital during 2007-2010 were analyzed and followed up. Drug sensitivity test were performed for the cultured Mycobacterium tuberculosis. Pyrazinamide resistant gene-pncA was amplified by PCR and analyzed for mutation by DNA sequencing. Results Genotypes of PZA resistance are consistent with the results of phenotype in all 22 mycobacterium tuberculosis strains. PZA resistance was detected positive in 11 cases,and negative in the other 11 strains. Among the 11 PZA resistant strains,4 strains were examined to be multi-drug resistant strains. Patients with PZA resistant occupied 70%(7/10) in the retreatment cases,and 80%(4/5) in the multi-drug resistant patients. Among all pyrazinamide resistant cases,through follow-up,it revealed that 2 cases died,3 cases accepted intermittent treatment(failure),and 6 cases were cured.However,among the PZA sensitive patients,all were recovered except one death. Conclusion Pyrazinamide resistance is common in the patients with recurrent tuberculosis and multi-drug tuberculosis,and they have a relatively worse prognosis compared with those with PZA sensitive tuberculosis.

Key words: Mycobacterium, Pyrazinamide, Drug resistance, Clinical feature