Influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice

Abstract

Abstract: Objective To compare the influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice. Methods 36 mice were enrolled in this study, and were randomly divided into propofol group (group P), ketamine group (group K), and control group (group C) with 12 cases each. Tuberculosis model was established in all mice 6 weeks before the test. Blood samples were taken from the tail vein at 30 minutes before operation, 1 day after the operation and 3 days post-operation, CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD4⁺/CD8⁺T lymphocyte ratio, and interferon gamma (IFN-γ) expression were calculated with flow cytometer. Lung slices and tubercle bacillus culture were observed at 3 weeks and 6 weeks after surgery with 6 mice in each group. Results Compared with group C, CD3⁺T lymphocyte, CD4⁺T lymphocyte, CD4⁺/CD8⁺T lymphocyte ratio, and IFN-γ expression in group P and group K decreased apparently after surgery, and tuberculosis bacterium count was higher (P<0.05). Compared with group K, CD3⁺T lymphocyte, CD4⁺T lymphocyte levels were higher but IFN-γ lower 3 days postoperative in group P (P <0.05), but there was no difference about postoperative tubercle bacillus replication in these two groups. Conclusion Both propofol and ketamine could inhibit postoperative T lymphocyte's function in tuberculosis mice, and tuberculosis bacterium number increase. However, the influence on T lymphocyte subtypes were not synchronously, meaning further researches are needed.

Key words: Propofol, Ketamine, Tubercle bacillus, Mice, T lymphocyte subtype

LIU Min-qiang, HAN Ya-kun, YU De-hui, MA Ming-fei, SHI Hao-wen, HE Ren-liang. Influence of propofol and ketamine on postoperative T lymphocyte subtype and tubercle bacillus replication in tuberculosis mice[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(2): 89-92.

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