The study of the expression of CXCL9 detected by ELISA in patients with tuberculosis and its application in clinic

Abstract

Abstract: Objective To detect the expression of CXCL9 in plasma from different groups of people by ELISA and to evaluate its potential in tuberculosis diagnosis. Method CXCL9 were detected by ELISA in the plasma from 16 cases of tuberculosis and 20 healthy controls, 20 cases of LTBI as well as 16 cases of TB. And ROC curve were drawn to evaluate the efficiency of CXCL9 in diagnosis of tuberculosis. Result The expression of CXCL9 in the plasma of those with tuberculosis was significantly higher than those are healthy controls of LTBI (P<0.001). The level of CXCL9 decreased significantly after anti- tuberculosis treatment. When CXCL9 was used to distinguish from tuberculosis to HC and tuberculosis, the value of AUC was 0.9125, with a sensitivity of 75% and a specificity of 90%. When CXCL9 was used to distinguish TB from LTBI, the value of AUC was 0.8188, with a sensitivity of 75% and a specificity of 80%. Conclusion The expression of CXCL9 in TB was high. It could be used as a biomarker in diagnosis of tuberculosis.

Key words: CXCL9, Tuberculosis, ELISA, Diagnostics, Biomarker

XIE Shu-zhi, SHENG Xing, CHEN Qi, ZHANG Ming-xia, DENG Guo-fang, YANG Qian-ting, DENG Qun-yi. The study of the expression of CXCL9 detected by ELISA in patients with tuberculosis and its application in clinic[J]. Electronic Journal of Emerging Infectious Diseases, 2017, 2(2): 93-95.

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