Abstract: Objective To investigate mycobacterium tuberculosis antigen specific IFN-γ response in tuberculosis pelvic inflammation patients（TPIP）by Elispot. Methods The levels of IFN-γ production in response to mycobacterium tuberculosis antigens in TPIP (n=63) and non-TPIP(n=19) were evaluated by an in-house Elispot. Result By using Elispot, the level of IFN-γin TPIP was much higher than the non-TPIP ,and there was statistical difference between the TPIP and the non-TPIP(p<0.05).The positive rate in Elispot observed in TPIP and non-TPIP was 80.9%,10.5% respectively. Conclusion It is practical to differentiate the tuberculosis pelvic inflammation patients from the non tuberculosis pelvic inflammation patients by measuring the Mtb. antigen-specific IFN-γ response in the peripheral blood using our in-house Elispot assay. It is reliable to use it to diagnosis the TPIP.

Key words: Tuberculosis pelvic inflammation, Elispot, Interferon-gamma