Establishment of rapid qPCR method for detection of five respiratory viruses based on microfluidic chip technology

Electronic Journal of Emerging Infectious Diseases ›› 2020, Vol. 5 ›› Issue (2): 95-99.

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Establishment of rapid qPCR method for detection of five respiratory viruses based on microfluidic chip technology

Ren Qin^1#, Zhang Xingyan^1#, Du Shouwen¹, Wang Yuhang¹, Yang Guilin¹, Lin Linhua², Dai Shaobo¹, Li Tiyuan¹, Jin Ningyi³, Li Chang³, Wu Shipin¹

1. Department of Infectious Diseases, the Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Guangdong Shenzhen 518020, China;
2. Department of Obstetrics, the Second Clinical Medical College, Shenzhen People's Hospital of Jinan University, Guangdong Shenzhen 518020, China;
3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun 130062, China

Received:2020-02-15 Online:2020-02-20 Published:2020-06-19

Abstract

Abstract: Objective To establish a rapid differential diagnostic method for five respiratory viruses (influenza A virus, influenza B virus, H1N1/H3N2 subtype influenza virus and measles virus). Methods We designed and synthesized specific probe primers for the five respiratory viruses respectively. Based on the latest microfluidic chip technology, the specificity, sensitivity and repeatability of the detection system of probe primers was evaluated by a rapid fluorescent quantitative PCR instrument with the rapid reaction system and innovative temperature control module, and the method was used to test some clinical samples.Results The probe primers of five viruses designed and synthesized have high specificity and sensitivity. Virus nucleic acid was extracted in 5 minutes, and the detection results were obtained in 30 minutes and had good repeatability. Conclusion A rapid and quantitative detection method for five respiratory viruses was established, which was expected to be used in the differential diagnosis and rapid detection of unknown respiratory virus infection.

Key words: Respiratory virus, Rapid quantitative detection, Microfluidic chip technology

Ren Qin, Zhang Xingyan, Du Shouwen, Wang Yuhang, Yang Guilin, Lin Linhua, Dai Shaobo, Li Tiyuan, Jin Ningyi, Li Chang, Wu Shipin. Establishment of rapid qPCR method for detection of five respiratory viruses based on microfluidic chip technology[J]. Electronic Journal of Emerging Infectious Diseases, 2020, 5(2): 95-99.

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Establishment of rapid qPCR method for detection of five respiratory viruses based on microfluidic chip technology

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