Isolation, identification and phylogenetic analysis of a dengue virus I

Electronic Journal of Emerging Infectious Diseases ›› 2020, Vol. 5 ›› Issue (2): 100-103.

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Isolation, identification and phylogenetic analysis of a dengue virus I

Wang Yuhang¹, Zhang Xingyan¹, Hua Qunyi², Ren Qin¹, Dai Shaobo¹, Li Tiyuan¹, Jin Ningyi³, Zhou Boping¹, Wu Shipin¹, Li Chang³, Du Shouwen¹

1. Department of Infectious Diseases, the Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Guangdong Shenzhen 518020, China;
2. Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Customs District P.R.C., Guangdong Shenzhen 518000, China;
3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130062, China

Received:2020-02-21 Online:2020-02-20 Published:2020-06-19

Abstract

Abstract: Objective To preliminarily understand the origin and evolution of the dengue virus isolated strain. Methods A dengue virus was isolated, and its serotyping and genome sequence was identified and analyzed. Especially, the phylogenetic analysis of envelope protein E was performed by bioinformatics methods, ResultsA serotype Ⅰof DENV isolated strain named as SZ905 was identified to be genotype Ⅰ. And the homology analysis of E gene showed that the nucleotide homology with Malaysia 0802atw strain, Singapore strain, Zhejiang 17-45 strain, YN176282 strain and BNJH 17108 strain were all above 99%, and the homology of E protein with the multiple strains such as NJX-14, Zhejiang 17-45, Malaysia 0802atw and 1208atw strains was all above 99.8% in amino acid level, indicating that these strains maybe originate from a same original strain. And the evolution of E protein sequence is stable and highly homologous with that of several strains isolated in Guangdong Province since 2002. Conclusion DENV-Ⅰ genotype Ⅰ in China has stable genetic evolution and high degree of conservation, which is conducive to the prevention and control of this serotype virus disease in recent years.

Key words: Dengue virus, Genotype, Phylogenetic analysis

Wang Yuhang, Zhang Xingyan, Hua Qunyi, Ren Qin, Dai Shaobo, Li Tiyuan, Jin Ningyi, Zhou Boping, Wu Shipin, Li Chang, Du Shouwen. Isolation, identification and phylogenetic analysis of a dengue virus I[J]. Electronic Journal of Emerging Infectious Diseases, 2020, 5(2): 100-103.

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Isolation, identification and phylogenetic analysis of a dengue virus I

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