Abstract: Objective To explore the effect of lysine methyltransferase 2E (KMT2E) knockdown on DENV virus Ⅱ (DENVⅡ) proliferation and preliminarily analyze the role of KMT2E in DENVⅡ proliferation. MethodAccording to the coding sequence of the kmt2e gene, three siRNAs were designed. 293T cells were transfected with these siRNAs to select the one with the highest efficiency in knockdown of KMT2E. RT-qPCR was used to analyze the proliferation of DENVⅡ after knockdown of KMT2E. ResultThe siRNA numbered as siKMT2E-1 possesses the highest knockdown efficiency of KMT2E protein, and can significantly inhibit DENVⅡ proliferation once transfected to 293T cells. Conclusion KMT2E maybe plays a positive role in the proliferation of DENVⅡ.

Key words: Dengue virus Ⅱ, Lysine methyltransferase 2E, Small interfering RNA