Abstract: Objective To explore the drug resistance of Helicobacter pylori(Hp) in Shenzhen and provide laboratory evidence for the selection of clinical antibiotics. Methods From January 2017 to January 2019, a total of 381 Hp positive participants were enrolled in this trial who had received ¹³C-urea breath test. They were examined by endoscopy and the gastric antrum/corpus mucosa were collected for Hp culture and antibiotic susceptibility test. The culture and antimicrobial results were counted and analyzed. Results A total of 315 Hp strains were isolated from 381 patients. The positive rate was 82.75% (315/381). The resistance rate of Hp to amoxicillin was 0% (0/231), rifampicin 2.22% (7/315), ciprofloxacin 23.81% (75/315), clarithromycin 32.69% (103/315), metronidazole 83.17% (262/315). 46 strains of Hp were sensitive to the above five antibiotics and the sensitivity rate was 14.60% (46/315). 269 strains of Hp were resistant to at least one antibiotic and the resistance rate was 85.40% (269/315), of which the single resistance rate was 51.43% (162/315), the double resistance rate is 26.35% (83/315), and the triple resistance rate is 7.62% (24/315).The gender and age will affect the resistance rate of Hp. The multi-drug resistance rate of Hp in male population increased with age, and the multi-drug resistance rate of elderly males was significantly higher than that of elderly females (P<0.05). Conclusions The metronidazole resistance rate of in Shenzhen is extremely high, which is not suitable for first-line treatment; The resistance rate of Helicobacter pylori amoxicillin is extremely low. Penicilin is recommended to be the first choice of treatment unless patient is allergic to the drug; Elderly males are more likely to have Hp resistance and multi-drug resistance. The history of antibiotic used should be carefully noted.

Key words: Helicobacter pylori, Antibiotic susceptibility test, Resistance rate, Antimicrobial agents