Preliminary analysis of the methylation level of CENPK gene promoter region in hepatocellular carcinoma patients with hepatitis B

doi:10.19871/j.cnki.xfcrbzz.2021.02.002

Electronic Journal of Emerging Infectious Diseases ›› 2021, Vol. 6 ›› Issue (2): 84-87.doi: 10.19871/j.cnki.xfcrbzz.2021.02.002

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Preliminary analysis of the methylation level of CENPK gene promoter region in hepatocellular carcinoma patients with hepatitis B

Le Xiaohua¹, Liu Zhuobing³, Zhang Xiangmei¹, Wang Haiyan³, Zeng Lijiao³, Zheng Juan³, Jiang Nan², Liu Weilong³

1. Department of Pathology, the Third People's Hospital of Shenzhen, Guangdong Shenzhen 518112, China;
2. Department of Laboratory Medicine, Shenzhen Overseas Chinese Town Hospital, Guangdong Shenzhen 518020, China;
3. Institute of Liver Diseases, the Third People's Hospital of Shenzhen, Guangdong Shenzhen 518112, China

Received:2020-12-29 Online:2021-05-31 Published:2021-06-24

Abstract

Abstract: Objective To compare the methylation level of CENPK gene promoter region in HCC and its paracancerous liver tissue of patients with hepatitis B, and to improve the level of early diagnosis and treatment of liver cancer. Methods The full-length genome sequence of CENPK gene was obtained from NCBI, and specific primers for typical CpG islands in CENPK gene promoter region were designed using Primer 5.0 software. Bisulfite sequencing PCR(BSP) was used to analyze and compare the methylation of 16 CG sites in CpG island of CENPK promoter region in 4 pairs of HCC and its corresponding paracancerous tissues. Results The methylation status of CpG island in CENPK promoter region was successfully screened and identified in 4 pairs of hepatocellular carcinoma and paracancerous tissues. The results of comparative analysis and sequencing showed that the methylation level of CpG Island in CENPK promoter region in 75% (3/4) hepatitis B patients was significantly lower than that in paracancerous tissues (P<0.05). Conclusions The abnormal high expression of CENPK gene in hepatocellular carcinoma is closely related to the level of DNA hypomethylation in the promoter region, suggesting that epigenetic mechanisms play an important role in the development of hepatocellular carcinoma.

Key words: Hepatocellular carcinoma, Centromere protein K, Methylation, High expression, Hepatitis B

Le Xiaohua, Liu Zhuobing, Zhang Xiangmei, Wang Haiyan, Zeng Lijiao, Zheng Juan, Jiang Nan, Liu Weilong. Preliminary analysis of the methylation level of CENPK gene promoter region in hepatocellular carcinoma patients with hepatitis B[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(2): 84-87.

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Preliminary analysis of the methylation level of CENPK gene promoter region in hepatocellular carcinoma patients with hepatitis B

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