Distribution of enterovirus genotypes in different populations and their pathogenic risk analysis

Abstract

Abstract: Objective To analyze the c distribution of enterovirus genotypes in healthy children, children with diarrhea, children with hand foot and mouth disease and children with encephalitis and meningitis and to assess their pathogenic risks. Methods Totally 273 fecal specimens of diarrhea group, 361 fecal specimens of healthy group from Hebei province, 320 fecal specimens from hand-foot-and-mouth disease group and 105 cerebrospinal fluid (CSF) samples of encephalitis meningitis group, 182 fecal specimens of healthy group from Hunan province were examined by real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube. The positive samples were further sequenced to determine the genotype of the enterovirus for analysis. Results The positive rate in 361 fecal specimens from healthy group of Hebei province was 16.34% (59/361). The 273 fecal specimens of diarrhea group showed a positive rate of 18.68% (51/273). The 320 fecal specimens of hand-foot-and-mouth disease group from Hunan showed a positive rate of 85.31% (273/320). The 105 CSF samples of encephalitis meningitis group had a positive rate of 24% (25/105). And the positive rate of 182 fecal specimens of healthy group was 18.68% (34/182). The positive rates in the 5 groups were statistically significant (χ²=466.4, P<0.0001). Conclusion The distribution of enterovirus in 5 different populations is different. Statistical analysis indicates that RV is a risk factor of diarrhea. EV71 and CVA are highly correlated with hand-foot-and-mouth disease. EV71 is correlated to encephalitis meningitis.

Key words: Enterovirus, Diarrhea, Hand foot mouth disease, Encephalitis

QIU Fang-zhou, SHEN Xin-xin, YAN Teng-fei, ZHOU Shuai-feng, ZHANG Yi, WANG Ji, HE Xiao-zhou, YANG Meng-jie, FENG Zhi-shan, MA Xue-jun. Distribution of enterovirus genotypes in different populations and their pathogenic risk analysis[J]. Electronic Journal of Emerging Infectious Diseases, 2018, 3(1): 8-11.

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