MiR-20a-5p negatively regulates Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis

doi:10.19871/j.cnki.xfcrbzz.2021.03.007

Electronic Journal of Emerging Infectious Diseases ›› 2021, Vol. 6 ›› Issue (3): 198-201.doi: 10.19871/j.cnki.xfcrbzz.2021.03.007

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MiR-20a-5p negatively regulates Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis

Zhang Gengwei¹, Ou Min², Zhou Xuefeng³, Tao Xiaoyu⁴, Fu Jiapeng⁴, Fu Xiangdong⁴

1. Department of Emergency, Shenzhen Third People's Hospital, Guangdong Shenzhen 518112, China;
2. Institute of Liver Disease, Shenzhen Third People's Hospital, Guangdong Shenzhen 518112, China;
3. School of Basic Medicine, Guangdong Medical University, Guangdong Dongguan 523808, China;
4. Department of Tuberculosis, Shenzhen Third People's Hospital, Guangdong Shenzhen 518112, China

Received:2021-03-31 Online:2021-08-31 Published:2021-10-11

Abstract

Abstract: Objective To analyze the effect of miR-20a-5p on Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis and explore the potential mechanism of mir-20a-5p gene regulating intracellular signaling pathway. Methods Human macrophages THP-1 were transfected with miR-20a-5p, miR-20a-5p-inh and negative control (NC) lentivirus, respectively. Green fluorescent protein (GFP) -positive THP-1 cells were selected by flow cytometry and infected with H37Ra. The expressions of AKT/JNK signaling pathway genes Akt1, Akt2, Jnk1 and Jnk2 were detected by qPCR.Western blotting (WB) was used to detect the expression of total Jnk2 and p-JNK in GFP⁺ THP-1 cells infected with H37Ra. Results The expression level of Jnk2 gene was decreased in THP-1 cells with miR-20a-5p overexpression, and increased when miR-20a-5p was inhibited.The expression trend of p-JNK2 (p54) at the protein level detected by WB was consistent with the qPCR results, that is, it was negatively correlated with the expression of miR-20a-5p. Conclusion miR-20a-5p negatively regulates Jnk2 expression in human macrophages infected with Mycobacterium tuberculosis at the level of gene expression and protein expression.

Key words: Macrophages, miR-20a-5p inhibitor, Jnk2 gene, Human macrophages THP-1, Mycobacterium tuberculosis

Zhang Gengwei, Ou Min, Zhou Xuefeng, Tao Xiaoyu, Fu Jiapeng, Fu Xiangdong. MiR-20a-5p negatively regulates Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis[J]. Electronic Journal of Emerging Infectious Diseases, 2021, 6(3): 198-201.

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MiR-20a-5p negatively regulates Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis

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