Expression and significance of zinc-finger antiviral protein in enterovirus 71 infected mice

doi:10.19871/j.cnki.xfcrbzz.2022.01.002

Abstract

Abstract: Objective To explore the expression level of host protein ZAP in enterovirus 71 (EV71) infected mice and its regulatory effect, and to provide data basis for antiviral treatment of EV71. Method The C57BL/6 mice were randomly divided into 4 groups, blank control group (Control group), infection group (EV71 group), infection + ZAP plasmid group (ZAP group) and infection + empty plasmid group (Mock group). In the latter three groups, EV71 was injected intraperitoneally to establish the infection model, and the mice in the plasmid injection group were injected with plasmids into the retro-orbital venous plexus to construct a ZAP overexpression mouse model. Hematoxylin-eosin (HE) staining, Western blot and quantitative real time polymerase chain reaction (Q-PCR) were used to detect mice. Viral load, pathological changes and expression levels of ZAP and VP1 in different tissues were detected. Conclusion ZAP plays an important role in the immune response to EV71 infection, suggesting that ZAP overexpression can improve the sensitivity of mice to EV71 virus and the resistance to viral inflammation. This finding provides new insights into the role of ZAP in virus-induced inflammatory disease.

Key words: Zinc-finger antiviral protein, Enterovirus 71, Mouse model, Interaction between virus and host

Xie Li, Wang Feng, Zhong Yuquan, Yang Hui. Expression and significance of zinc-finger antiviral protein in enterovirus 71 infected mice[J]. Electronic Journal of Emerging Infectious Diseases, 2022, 7(1): 6-11.

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