Application of extractive electrospray ionization mass spectrometry in epidemiology

doi:10.19871/j.cnki.xfcrbzz.2022.04.020

Abstract

Abstract: Extractive electrospray ionization mass spectrometry (EESI-MS) combines the advantages of electrospray ionization and liquid-liquid extraction. EESI-MS can be used for real-time mass spectrometry analysis of complex samples, featuring the characteristics of strong tolerance of biomatrices, good stability, high sensitivity, and wide applicability. It has become an effective mass spectrometry method for in vivo analysis, in-situ analysis, and long-distance analysis. For liquidand gas, EESI-MS can be directly carried out to obtain analytical data. For actual samples such as colloids, heterogeneous mixtures and living plant surface, EESI-MS technology can adopt neutral gas desorption sampling, which can realize mild mass spectrometry analysis without chemical pollution, biological stress, and obvious damage, and has no obvious interference to the physiological or pathological state of animals and plants. More importantly, the improved internal extraction electrospray ionization (iEESI) MS technology can directly and quantitatively achieve trace amounts of trace substances in tissue samples without pretreatment of homogenate and extraction. Therefore, EESI-MS technology has the characteristics of powerful function and wide application. In recent years, EESI-MS has been widely used in epidemiology, which includes the analysis of infectious diseases and chronic diseases, the detection of harmful substances affecting human health in the fields of environment, labor and food. EESI-MS has special advantages in the molecular diagnosis of infectious diseases and chronic diseases. Due to the limitation of length and theme, this paper mainly reviews the application of EESI-MS technology in epidemiology to promote the exchange and development of related fields.

Key words: Extractive electrospray ionization mass spectrometry, Epidemiology, Infectious diseases, Chronic disease, Diagnosis of disease

Zhai Xingying, Long Zi, Zhang Xinglei, Zhu Tenggao, Liu Kun, Xu Jiaquan. Application of extractive electrospray ionization mass spectrometry in epidemiology[J]. Electronic Journal of Emerging Infectious Diseases, 2022, 7(4): 86-90.

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