The role of macrophage MAC387 in the inflammation and fibrosis of hepatic alveolar echinococcosis of human cases

doi:10.19871/j.cnki.xfcrbzz.2023.01.004

Abstract

Abstract: Objective To investigate the correlation of MAC387 with liver inflammation and fibrosis in patients infected with hepatic alveolar echinococcosis (AE). Method Liver tissues were collected from 32 hepatic AE patients with surgical removal. Each of the liver tissues was divided into two section groups, close to AE lesion (CLT) and distal from AE lesion(DLT). Hematoxylin-eosin (H&E) and Sirius red staining were employed to assess the pathological inflammatory changes and collagen deposition in these patients' livers, respectively determined by the inflammatory grading scores and fibrotic staging scores (GS scores). Immunohistochemistry was used to detect the expressions of MAC387 in the two section groups. The patients were divided into two groups, MAC387 high-level group and MAC387 low-level group. The correlation of MAC387 with major biochemical parameters associated with liver functions and their changes before and after operation were analyzed. RT-qPCR was employed to measure the mRNA expression of MAC387 in the two liver section groups. Result Compared to the DLT, the level of MAC387 was significantly increased in CLT sections(P<0.0001). Additionally, patients with higher inflammatory grading scores and fibrotic staging scores had more MAC387-positive cells in the liver than patients with lower scores (P<0.001). In the MAC387 high-level group, MAC387 had a positively correlation with ALT and AST (ALT：r=0.523, P=0.013;AST：r=0.503, P=0.017), and there was no significant correlation with GGT, ALP and Alb（P>0.05). In the MAC387 low-level group, there was no correlation between MAC387 and ALT, AST, GGT, ALP, Alb. After 9 days of operation, GGT in MAC387 low-level group was significantly higher than MAC387 high-level group（P<0.05）. RT-qPCR showed that MAC387 expression was higher in the CLT section group than it in the DLT(P<0.05). Conclusion MAC387 is highly expressed in CLT in the patients with hepatic AE, and the infiltration of MAC387 positive cells have higher score of inflammation grade and fibrosis stage, suggesting that MAC387 may play a role in aggravating liver inflammation and fibrosis in AE patients.

Key words: Hepatic alveolar echinococcosis, MAC387⁺ monocyte-derived macrophages, Liver inflammation and fibrosis

CLC Number:

R575
R53

Zhang Jingyi, Tian Fengming, Jiang Tao, Qi Xinwei, Li Jiajun, Min Hongyue, Madinaimu•Aibibula, Zhou Xuan, Liu Yumei, Ma Xiumin. The role of macrophage MAC387 in the inflammation and fibrosis of hepatic alveolar echinococcosis of human cases[J]. Electronic Journal of Emerging Infectious Diseases, 2023, 8(1): 19-25.

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