The analysis of mycobacterial infection and drug resistance among children in Sichuan

doi:10.19871/j.cnki.xfcrbzz.2023.01.008

Abstract

Abstract: Objective Understand the situation of mycobacterial infection and drug resistance in children in Sichuan, provide data support for the prevention and management of tuberculosis in children in the region, and provide help for clinical treatment. Method From January 2019 to March 2021,various specimens of children younger than 15 years old admitted to the tuberculosis area and tuberculosis clinic of Chengdu Public Health Clinical Medical Center were isolated and cultured for mycobacteria, and the positive strains were cultured for silver micropores Plate method (48 holes) rapid drug susceptibility test, Microplate method drug susceptibility test includes strain identification (TCH, PNB) and streptomycin (SM), isoniazid (INH), rifampicin (RFP), ethambutol (EMB), rifapentin (RFT), levofloxacin (LFX), amikacin (AMK), capreomycin (CPM), prothionamide (PTO), lectra pasiniazid (PI), moxifloxacin (MFX), P-Amino Water Susceptibility results of 16 drugs including salicylic acid (PAS), clarithromycin (CLR), rifabutin (RFB), kanamycin (KAN), and clofazimine (CFZ). The distribution, drug resistance and epidemiological characteristics of mycobacterial culture-positive children were retrospectively analyzed. Result Among the 200 strains of mycobacteria in children, 194 strains (97.00%) of Mycobacterium tuberculosis (MTB) were found, and 6 strains (3.00%) of nontuberculous mycobacteria (NTM). The results of MTB drug susceptibility showed that 43 of the 194 MTB strains were resistant to at least one anti-tuberculosis drug, with a total resistance rate of 22.16%; 10 strains were resistant to INH and RFP (MDR) at the same time, with a multidrug resistance rate of 5.15%, no extensively drug-resistant (XDR) strains were found. Mono-resistance was mainly mono-resistance to INH, and multi-resistance was mainly RFP+RFB+RFT and INH+PI. The resistance rankings were INH (12.37%), RFP (9.79%), RFT (9.79%), RFB (7.22%), PI (5.67%), SM (4.12%), PAS (2.58), CPM (2.06) main. There were significant differences in ethnicity (Han nationality), age (7-11 years old and 12-14 years old), and residence (Tibetan area) between the sensitive group and the resistant group (P<0.05). Conclusion The INH drug resistance rate of pediatric tuberculosis patients in Sichuan is the highest, and children in Tibetan areas in Sichuan are more likely to develop drug-resistant tuberculosis. This study provides a theoretical basis for the prevention and treatment of pediatric tuberculosis in Sichuan.

Key words: Children, Mycobacterial infection, Rapid microplate test, Epidemiology, Drug-resistant tuberculosis

CLC Number:

R725.1

Xu Yuanhong, Wu Zhangjun, Luo Mei, Zhu Ma, Wang Dongmei, Zeng Peibin. The analysis of mycobacterial infection and drug resistance among children in Sichuan[J]. Electronic Journal of Emerging Infectious Diseases, 2023, 8(1): 39-43.

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