Immune responses induced by dengue serotype Ⅱ virus DNA vaccine are not associated with gender in mice

Abstract

Abstract: Objective To investigate the difference in protective efficacy of dengue serotype Ⅱ virus DNA vaccine pVAX1-D2ME between male and female mice. Methods Male and female mice were separately immunized with pVAX1-D2ME plasmids. Sera were obtained before each immunization and after the last immunization to evaluate the humoral immune responses. Two weeks after the last immunization, the mice were challenged with the dengue serotype Ⅱ virus (DENV2) to observe the difference of survival rate between male and female mice. Results Male and female mice showed no significant differences in the levels of specific antibodies produced by this vaccine. The immune response showed a Th1-type tendency. All the mice immunized showed complete protection against the lethal dose of virus with 100% survival rate. Conclusion sThe pVAX1-D2ME could induce strong immune response and effective protection against DENV2 in mice, and these effects are not related to the gender of mice.

Key words: Dengue virus, DNA vaccine, Gender, Humoral immune responses, Protection

WANG Ran, GAO Na, AN Jing. Immune responses induced by dengue serotype Ⅱ virus DNA vaccine are not associated with gender in mice[J]. Electronic Journal of Emerging Infectious Diseases, 2018, 3(2): 98-101.

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