Current Issue

2021 Vol.6 No.3 Published:31 August 2021

The Communist Party of China has been working for the people's health for 100 years

The era significance of Yujiang's spirit of "farewell to the God of Plague" for schistosomiasis control Huang Guohua, Shu Qianqian, Huang Yiran 2021, 6 (3):  167-171.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.001 Abstract ( 542)    PDF (953KB) ( 709 )    Schistosomiasis is an endemic disease caused by adult worms of Schistosoma japonicum. It mainly distributes in the middle and lower reaches of the Yangtze River and the south of the Yangtze River Basin, and poses a great threat to people's life and health. This paper briefly reviews the hard process of Yujiang people's elimination of Oncomelania and overcoming schistosomiasis under the leadership of the Communist Party of China, aiming to elaborate the formation, development and practical significance of Yujiang's schistosomiasis control spirit. The leadership of the Communist Party of China is the decisive factor for the elimination of schistosomiasis in Yujiang, and the grass-roots party organizations play an important role in the final victory. Yujiang's great campaign to eliminate schistosomiasis has profoundly revealed the incomparable superiority of the socialist system in China, it has important reference significance for the prevention and control of infectious diseases under the leadership of the Communist Party of China.

Original Articles

Artificial intelligence quantitative analysis reveals the effect of interleukin-6 injury in 2019 novel coronavirus B.1.617.2 Gan Qingxin, Liu Jinxin, Yang Yanhong, Hu Tianli, Zhang Lieguang, Huang Deyang, Li Meiyu 2021, 6 (3):  172-176.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.002 Abstract ( 323)    PDF (1092KB) ( 520 )    Objective To investigate the correlation of lung injury caused by interleukin-6 (IL-6) in 2019 novel coronavirus (2019-nCoV) B.1.617.2. It is suggested that the level of IL-6 should be detected in time and intervention should be carried out in advance in order to reduce the risk of severe pneumonia. Methods A retrospective study was conducted to collect 123 patients infected with 2019-nCoV B.1.617.2 from the Guangzhou Eighth People's Hospital, Guangzhou Medical University, in May 21 to June 21, 2021. There were 105 mild cases and 18 severe cases. The clinical characteristics, inflammatory index levels, IL-6 and artificial intelligence(AI) lung injury degree of the two groups were analyzed. Results The mean value of age, peak value of procalcitonin (PCT), peak value of serum IL-6 and peak value of pulmonary injury measured by AI in patients with 2019-nCoV B.1.617.2 strain were higher than those in patients with mild disease group (P<0.05). The mean value of serum IL-6 in patients with severe disease group was （197.28±105.70）pg/ml. The peak value of lung injury measured by AI accounted for （36.21±14.91）%, and the main imaging features of lung injury were multiple ground glass opacity and consolidation in bilateral lungs. Conclusion High expression of IL-6 is a potential immune risk factor for lung injury in patients with 2019-nCoV B.1.167.2 strain. AI quantitative analysis helps to determine the severity of pneumonia in patients, intervention as early as possible. In order to reduce the risk of severe pneumonia.

Investigation, analysis and diagnosis of an imported COVID-19 case with ovale malaria in Guangdong Province Lu Qian, Gao Shitong, Liu Qu, Zhang Zhongming 2021, 6 (3):  177-180.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.003 Abstract ( 378)    PDF (968KB) ( 626 )    Objective The purpose is to provide references for malaria prevention and control strategy during the COVID-19 epidemic through analyzing the pathogenesis, diagnosis and treatment of the city's first imported COVID-19 case with ovale malaria. Methods The method is to carry out epidemiologic investigation on the suspected case of new crown-combined malaria who entered Shenzhen for quarantine on December 19, 2021 through the active case investigation of the Longgang District Health Station. The samples of the case were collected for testing and sent to the superior department for review. Descriptive epidemiological method was used for analysis. Investigation Results The case involves an overseas worker (male, 47 years old) who returned to China from Kenya on December 19, 2020 and was transferred to a "health station" for centralized isolation via the closed-loop management system after he landed on the airport. He was first detected positive for novel coronavirus at a nucleic acid test on December 25 and was confirmed to be positive for novel coronavirus after re-checked by the Shenzhen Center for Disease Control and Prevention. Meanwhile, the patient was tested negative in malaria antigen rapid diagnostic test (RDT) and positive for plasmodium in microscopic examination, and after fluorescence PCR test, he was confirmed to be infected with ovale malaria. Conclusion sBased on the results of the epidemiologic investigation, clinical symptoms and lab tests, the case was confirmed to be an asymptomatic COVID-19 patient with ovale malaria.

Pharmacological value of Lonicera japonica flos on the treatment of mild COVID-19 patient Guo Jinhan, Chen Jianlin, Ge Lanlan, Wan Haoqiang, Yang Tianqing, Miao Yuyang, Xiao Lingyun, Ling Yunzhi, Zeng Xiaobin 2021, 6 (3):  181-188.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.004 Abstract ( 829)    PDF (1830KB) ( 1099 )    Objective The purpose of this study was to investigate the potential pharmacological value of Lonicera japonica flos extract in treating mild COVID-19, and to provide a new idea of TCM prevention and treatment for mild COVID-19. Methods TCMSP database was used to screen out the active components and action targets of honeysuckle. SwissADME and pkCSM databases were used to predict pharmacokinetics of constituents.The GEO database collected transcriptional sequencing data for mild COVID-19. Data quality control, DEGs identification and enrichment analysis were realized by R tool. STRING and Cytoscape constructed protein interaction networks and screened core DEGs using cytoHubba. Molecular docking was performed using PyMOL software. Results The 7 active ingredients of Honeysuckle had good oral absorption and utilization and drug-like characteristics.There were three targets associated with mild COVID-19: COL20A1, NOS2, and DUOX2. There were 5 common enrichment pathways, including peroxides metabolism, reactive oxygen biosynthesis and metabolism, heme, tetrapyrrole binding and so on. Conclusion Honeysuckle had the characteristics of multi-target and multi-pathway action in the treatment of mild COVID-19. The possible mechanism of action was to intervene in mild COVID-19 by regulating reactive oxygen species and heme related processes during the development of mild COVID-19 through NOS2 and DUOX2 expression.

Evaluation of the false positive results of IgG and IgM antibodies to 2019-nCoV Wei Lanlan, Yu Siyang, Zhu Xujie, Peng Yiman, Lin Baoyue, Zhang Yaxi, Zhang Juanjuan, Liao Mingfeng, Lin Jingyan, Zhang Zheng 2021, 6 (3):  189-192.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.005 Abstract ( 473)    PDF (903KB) ( 763 )    Objective To investigate the false positive results of serum IgG and IgM specific to the 2019 novel coronavirus,so as to give full play to the value of antibody detection in diagnosis and prevention and control of COVID-19. Methods The 2019-nCoV nucleic acid detection results, 2019-nCoV vaccine injection history and other relevant clinical information were collected from 9034 hospitalized patients from August 2020 to February 2021. Serum specimens were collected and the levels of IgG and IgM were detected by magnetic particle chemiluminescence. The false positive rate of IgG and IgM and its influencing factors were analyzed. Results ① The false positive rates of IgG and IgM were 0.75% and 0.34%, respectively. ② The false positive results of IgG（P=0.6098）and IgM（P=0.8288） were not correlated with gender. False positive result of IgG was not correlated with age（P=0.9130）, while the false positive result of IgM was affected by the age of the patients（P=0.0022）. The false positive rate of IgM was significantly increased in the patients with age older than 45. ③In IgG and IgM antibody false positive patients, infected patients accounted for the highest proportion. Conclusion The false positive rates of IgG and IgM detected by magnetic particle chemiluminescence are low. Age over than 45 years is an influencing factor to false positive results of IgM.

Meta analysis of correlation between chest CT features and clinical severity of 2019 novel coronavirus pneumonia patient Yin Run, Xi Yan, Liu Xinjiang, Fang Hui 2021, 6 (3):  193-197.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.006 Abstract ( 384)    PDF (1068KB) ( 566 )    Objective Meta analysis was used to explore the relationship between chest CT features and clinical severity of 2019 novel coronavirus pneumonia, so as to provide basis for screening and diagnosis of 2019 novel coronavirus pneumonia patient. Methods "Embase" "PubMed" "Cochrane Library" was retrieved from December 27, 2019 to December 1, 2020, and the key words of 2019 novel coronavirus pneumonia were searched. The standard of the selected papers was in the same chest CT research literature, including non severe and severe patients. The data included chest CT lesion distribution, lesion composition, signs, mediastinum and pleural involvement. After data collection, Stata 12.0 software was used for data analysis. Results A total of 4105 people, 2639 in the non severe group and 1466 in the severe group were included in 23 articles. The statistical results showed that the CT features more likely occured in severe group were: consolidation, coexistence of ground glass opacity and consolidation, crazy-paving pattern, air bronchogram, bronchial wall thickening,traction bronchiectasis,parenchymal band,vascular enlargement, lymphadenopathy,bilateral distribution, central distribution, coexistence of peripheral and central distribution,right upper lobe, right middle lobe, right lower lobe and left lower lobe distribution. The non severe group was more likely to have nodules, while the severe group and the non severe group had no significant difference in CT signs, including ground glass opacity, interlobular septal thickening, reticular sign, peripheral distribution and left upper lobe distribution. Conclusion Lesion distribution, lesion composition and CT signs were different in non severe group and severe group of 2019 novel coronavirus pneumonia. In severe group, there were more consolidationlesions, ground glass opacity and consolidation mixed lesions, crazy-paving pattern, vascular enlargement, pleural effusion and so on.

MiR-20a-5p negatively regulates Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis Zhang Gengwei, Ou Min, Zhou Xuefeng, Tao Xiaoyu, Fu Jiapeng, Fu Xiangdong 2021, 6 (3):  198-201.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.007 Abstract ( 424)    PDF (1001KB) ( 698 )    Objective To analyze the effect of miR-20a-5p on Jnk2 gene expression in human macrophages infected with Mycobacterium tuberculosis and explore the potential mechanism of mir-20a-5p gene regulating intracellular signaling pathway. Methods Human macrophages THP-1 were transfected with miR-20a-5p, miR-20a-5p-inh and negative control (NC) lentivirus, respectively. Green fluorescent protein (GFP) -positive THP-1 cells were selected by flow cytometry and infected with H37Ra. The expressions of AKT/JNK signaling pathway genes Akt1, Akt2, Jnk1 and Jnk2 were detected by qPCR.Western blotting (WB) was used to detect the expression of total Jnk2 and p-JNK in GFP+ THP-1 cells infected with H37Ra. Results The expression level of Jnk2 gene was decreased in THP-1 cells with miR-20a-5p overexpression, and increased when miR-20a-5p was inhibited.The expression trend of p-JNK2 (p54) at the protein level detected by WB was consistent with the qPCR results, that is, it was negatively correlated with the expression of miR-20a-5p. Conclusion miR-20a-5p negatively regulates Jnk2 expression in human macrophages infected with Mycobacterium tuberculosis at the level of gene expression and protein expression.

Matching analysis of propensity scores for metformin and tuberculosis infection risk in T2DM patient Tan Yuncai, Yuan Yuan, Yang Shuangxi, Yang Xue 2021, 6 (3):  202-205.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.008 Abstract ( 351)    PDF (911KB) ( 428 )    Objective To investigate the correlation between metformin and the risk of pulmonary tuberculosis (TB) infection in patients with type 2 diabetes (T2DM) and provide reference for the treatment of T2DM patients. Methods 179 patients with T2DM who were treated in the Department of Infectious Diseases of Enshi Minority Hospital from January 2019 to December 2019 were selected, of which 58 patients were diagnosed with TB and 121 patients did not suffer from TB. Collect the patient's use of metformin, clinical data and laboratory examination data. The two groups of patients were matched by propensity score, and then the correlation between metformin use rate and the occurrence of TB in T2MD patients after matching was analyzed by Logistic regression. Results After the propensity score matching, the use rate of metformin in the TB group was significantly lower than that in the non-TB group, and the TB exposure history and Cr were significantly higher than those in the non-TB group（χ2=9.375、4.529,t=2.112,P=0.002、0.033、0.036）. Metformin use (OR=0.368, 95% CI= 0.167-0.811) is an independent protective factor that affects the occurrence of TB, and the history of TB exposure (OR=3.007, 95% CI=1.026-8.815) is a risk factor that affects the occurrence of TB （P=0.013、0.045）. Conclusion Long-term use of metformin in T2DM patients can reduce the risk of TB. Consider the combination of metformin treatment for T2DM patients with high TB risk, thereby reducing the risk of TB.

Analysis of influencing factors of group B hemolytic streptococcus infection in perinatal women and the influence of intrapartum preventive treatment on pregnancy outcome Tang Minyun, Wen Qionghua, Xiong Chong 2021, 6 (3):  206-209.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.009 Abstract ( 448)    PDF (905KB) ( 572 )    Objective To analyze the influencing factors of group B hemolytic streptococcus (GBS) infection in perinatal pregnant women and the effect of intrapartum antibiotic prophylaxis (IAP) on pregnancy outcome, so as to provide theoretical basis for eugenics. Methods 1850 pregnant women in late pregnancy who were established in Wanning people's Hospital from June 2018 to June 2020 were selected. According to the results of GBS detection of vaginal secretion, they were divided into GBS positive group and GBS negative group. The influencing factors of GBS infection in reproductive tract were analyzed by logistic regression. GBS positive patients were divided into GBS positive treatment group and GBS positive non treatment group according to the wishes of pregnant women.The pregnancy outcomes of the three groups were compared. Results ①Among 1850 pregnant women, 124 were GBS positive, and the positive rate of GBS was 6.70%. Logistic regression analysis showed that abortion history, vaginitis and gestational diabetes mellitus were risk factors for GBS infection. ②There was no significant difference in postpartum pelvic inflammatory disease, amniotic fluid pollution and postpartum hemorrhage among the three groups of pregnant women(P>0.05); The preterm birth rate of GBS positive untreated group was higher than that of GBS negative group (P<0.017). There were significant differences in the incidence of premature rupture of membranes among the three groups (P<0.017). There was no significant difference in birth weight among the three groups (P>0.05); there was significant difference in Apgar score among the three groups (P<0.05); the incidence of neonatal infectious pneumonia in GBS positive treatment group and GBS positive untreated group was higher than that in GBS negative group, and the incidence of neonatal sepsis in GBS positive untreated group was higher than that in GBS negative group (P<0.017). Conclusion The risk factors of GBS infection in pregnant women during perinatal period are abortion history, vaginitis and gestational diabetes. IAP can effectively improve the pregnancy outcome of pregnant women infected with GBS.

Detection of Helicobacter pylori by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Zhang Shuzhen, Li Xuehong, Huang Haiyan, Chen Haiting 2021, 6 (3):  210-214.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.010 Abstract ( 325)    PDF (927KB) ( 512 )    Objective Establish the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)'s ability of identifying Helicobacter pylori (Hp) and evaluate the effect of Hp-resistant strains on the results. Methods The Hp strains which were verified by traditional methods, drug resistance tests and confirmed by full gene sequencing were used for building MALDI-TOF MS library. 10 drug-sensitive Hp strains from the library were randomly collected to establish Hp identification Super Spectrum. Then, 201 Hp strains obtained from newly collected gastric mucosal biopsy samples were tested and evaluated by the MALDI-TOF MS. They were verified by culture identification, drug resistance test, and complete gene sequencing as well. Results The 201 Hp strains were tested by the MALDI-TOF MS. 196 strains of them were positive. The positive rate of MALDI-TOF MS was 97.5% (196/201). 33 strains of them were drug sensitive, which were all positive and the positive rate was 100% (33/33); 168 stains were drug-resistant. 163 strains of them were positive and the positive rate was 97.0% (163/168). There was no statistically significant difference between the two groups (P>0.05). Conclusion The identification of Hp by MALDI-TOF MS is accurate, which can greatly shorten the time of culture and shows a good clinical application value. The Hp-resistant strains have no significant effect on the identification accuracy.

MIRU-VNTR genotyping characteristics and transmission analysis of clinical isolates of tuberculosis in Shenzhen schools in 2018 Xie Li, Yang Zheng, You Xinxin, Fu Jiaolan, Wang Feng, Yang Hui 2021, 6 (3):  215-219.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.011 Abstract ( 652)    PDF (1011KB) ( 613 )    Objective Use variable-number tandem repeat (VNTR) genotyping technology to monitor the prevalence of tuberculosis in schools, and understand the genetic polymorphisms and recent transmission of tuberculosis clinical isolates in Shenzhen schools in 2018. Methods Genomic DNA was extracted from tuberculosis patients from shenzhen center for chronic disease control and designated hospitals, and 12 MIRU-VNTRs sites were detected and analyzed by Polymerase Chain Reaction (PCR).Online analysis software MIRU-VNTRS and MEGA7.6 were used for genotyping cluster analysis and phylogenetic tree mapping. Results A total of 63 tuberculosis culture-positive strains were collected in 2018, including 52 strains (82.5%) from student patients and 11 strains (17.5%) from faculty patients. The incidence of TB among students was 44.23% in high school/vocational school and 40.38% in university, while the incidence of TB among teaching staff was mainly in kindergarten (54.5%). The 63 strains of Mycobacterium tuberculosis were divided into 59 genotypes, including 56 unique genotypes and 3 gene clusters. Among them, 43 strains (68.3%) were Beijing genotype and 17 strains (27.0%) were non-Beijing genotype. In the three schools were found in two patients infected with strains of TB cases, and the results of the tuberculosis epidemiological investigation were determined to be independent infection, intra-school transmission and family cluster epidemic. Conclusion In 2018, the genotype diversity of tuberculosis strains in Shenzhen schools was high, and there were no more than 2 cases of clustered epidemics. The status of prevention and control of tuberculosis epidemics in schools is good. The MIRU-VNTRS homology identification technology can be used for routine monitoring and emergency detection of molecular epidemiological investigation of clustered tuberculosis in population.

Analysis of intestinal infection and drug resistance in 381 AIDS patients Xie Luman, Huang Kui, Qin Shanfang 2021, 6 (3):  220-224.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.012 Abstract ( 453)    PDF (913KB) ( 530 )    Objective To investigate the immune status, flora distribution and their resistance of intestinal pathogenic bacterium in patients with AIDS-related chronic diarrhea, and to provide a basis for clinical medication selection. Methods A total of 381 AIDS patients with chronic diarrhea who hospitalized in Longtan Hospital of Guangxi Zhuang Autonomous Region from January 2016 to October 2020 were collected for flora analysis and drug sensitivity. Further, these patients were evaluated immune status by analyzing the contents of CD4+T and CD8+T lymphocytes. To compare the immune status of AIDS patients with chronic diarrhea, 400 AIDS patients who without opportunistic infection were enrolled into the control group to assess immune status via performing flow cytometry as well. Results There were 357 cases found fungi in excrement, the types of fungi including183 cases of Candida albicans (51.26%); 100 cases of Candida glabrata (28.01%); 37 cases of Candida tropicalis (10.66%); 24 cases of Candida krusei (6.72%); 13 cases of Candida paraglabrata (3.64%); among of these fungus infection patients, some of them simultaneously infected with bacterial, there were 18 cases (4.72%) with intestinal Mycobacterium infection and6 cases (1.57%) with intestinal Salmonella infection. In the cases of fungal infection, the sensitivity of each fungus to amphotericin B, fluconazole, itraconazole, voriconazole and 5-fluorouracil was significant difference (P<0.05). Candida albicans infection is the most common and is sensitive to all five antifungal drugs. The drug resistance rate of those with tubercle bacillus cultured in stool was 38.89%~66.67%. The susceptibility to commonly used antibiotics in feces cultured with Salmonella infection was 30.33%~100.00%. AIDS patients with an intestinal bacterial infection will have lower contents of immune cells than without intestinal infection. The results of flow cytometry showed that CD4+T lymphocytes 33.00(10,90) /μl, CD8+T lymphocytes 351.00(201,577)/μl, and CD4+T/CD8+T 0.11 (0.04,0.22) for test group. Control group CD4+T lymphocytes 222.00(125,334)/μl, CD8+T lymphocytes 744.00(524,1144)/μl, CD4+/CD8+ 0.28(0.16,0.43), two groups of CD4+T, CD8+T lymphocytes and CD4+T/CD8+T were significantly different (P<0.01). Conclusion The weakened immunity of AIDS patients leads to intestinal barrier dysfunction, bacterial translocation, imbalance of intestinal flora, and ultimateintestinalinfection. And the choice of antibacterial drugs should base on drug sensitivity and ensure the treatment efficacy.

Current situation of imipenem drug use and correlation analysis between drug resistance and common gram-negative bacteria Chen Gang, Yang Yong, Luo Anjing, Zhang Gang, Feng Jiao 2021, 6 (3):  225-229.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.013 Abstract ( 475)    PDF (972KB) ( 591 )    Objective To understand the current situation of imipenem drug use and correlation analysis between drug resistance and common gram-negative pathogenic bacteria,to provide reference for clinical control of pathogen resistance. Methods Statistics from Sichuan Linshui county People's Hospital during the period of 2017–2018 imipenem drug use intensity per hundred beds and day, correlation between imipenem drug use intensity and drug resistance of common gram-negative pathogenic bacteria(Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Haemophilus influenzae, Acinetobacter baugh) were analyzed. Results DDDs and AUD of imipenem increased significantly in 2018 while compared with 2017, and the difference was significant (P<0.05). In 2017, 327 gram-negative pathogenic bacteria were cultured and isolated. Seven hundred and sixty-five gram-negative pathogenic bacteria were cultured and isolated in 2018. One thousand and ninety-two gram-negative pathogenic bacteria were cultured and isolated in 2017–2018. Pseudomonas aeruginosa infection was mainly distributed in respiratory tract, eye-nose-mouth. Klebsiella pneumoniae, enterobacter cloacae, haemophilus influenzae and acinetobacter baugh were mainly distributed in respiratory tract.Escherichia coli were mainly distributed in eye-nose-mouth, urinary tract, blood and respiratory tract.The drug resistance rate of gram-negative pathogenic bacteria to imipenem was 6.32%, among which pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, enterobacter cloacae and haemophilus influenzae had lower drug resistance rate to imipenem, while Acinetobacter baumannii had higher drug resistance rate to imipenem. The drug resistance rate of pseudomonas aeruginosa to imipenem in 2018 was significantly higher than that in 2017, and the drug resistance rate of acinetobacter bauxis to imipenem was significantly lower than that in 2017, P<0.05. DDDs and AUD were positively correlated with pseudomonas aeruginosa, klebsiella pneumoniae and haemophilus influenzae (P<0.05), while escherichia coli was not significantly correlated (P>0.05). Conclusion It has an obvious relationship between imipenem dosage and imipenem resistance of gram-negative pathogenic bacteria. Clinicians should pay attention to the rational use of imipenem to avoid the emergence of drug-resistant bacteria.

Chest radiographic features and differential diagnosis of respiratory syncytial virus in children Xie Nan'ai, Zhou Jie, Xiang Ziyun 2021, 6 (3):  230-233.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.014 Abstract ( 369)    PDF (1388KB) ( 836 )    Objective To investigate the chest X-ray features of children with respiratory syncytial virus pneumonia（RSV）, and to evaluate the value of chest X-ray in the diagnosis and differential diagnosis of respiratory syncytial virus pneumonia. Methods To retrospectively analyze the clinical and imaging data of children with respiratory syncytial virus pneumonia diagnosed by nasal swab nucleic acid detection, summarize the characteristic manifestations of children with respiratory syncytial virus pneumonia on chest X-ray, and identify the common viral pneumonia related literature in children. Results In the chest X-ray of 153 children with respiratory syncytial virus pneumonia, 102 cases (66.7%) showed multiple patchy hyperdense shadows distributed along the bronchovascular bundle, mainly involving the inner zone of bilateral lung fields (57 cases, 37.2%), and 6 cases (3.9%) showed pulmonary consolidation, mostly distributed in the right upper lung field and bilateral lower lung fields; 42 cases (27.5%) only showed bronchitis; No pleural effusion was found in all patients. Conclusion Although the diagnosis of respiratory syncytial virus pneumonia in children should be confirmed by etiology, the radiation dose of chest X-ray is low and easy to operate, which plays an irreplaceable role in the detection, distribution and severity evaluation of lung lesions.

An investigation outbreak of legionnaires' disease in a factory He Zhiming, Huang Fuye, Zhong Weike 2021, 6 (3):  234-237.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.015 Abstract ( 493)    PDF (971KB) ( 485 )    Objective To investigate an outbreak in a factory, determine the pathogen, source of infection and risk factors of transmission, and provide scientific basis for epidemic prevention and control. Methods From November 12 to 20, 2019, Bao'an District Songgang Station for Health Prevention of Shenzhen city carried out case investigation, occupational health and environmental health investigation on a factory suspected of Legionella disease outbreak according to the on-site epidemiological investigation steps, and collected samples for laboratory testing. Results from November 12 to 20, 2019, there were 45 cases, including 4 suspected cases .36 clinical cases and 5 confirmed cases, with an attack rate of 10.98% (45/410). The main symptoms were headache (86.67%), fatigue (77.78%), chest tightness (73.33%), dizziness (71.11%), etc. The white blood cell count was (9.81~30.29)×109/L, neutrophil count (7.35~28.15)×109/L. The onset time of the first case was 13:30 on November 15, and that of the last case was 21:10 on November 15, which was consistent with the characteristics of point source exposure. All of them are day workers in the assembly workshop on the second floor of the third factory. Among them, the morbidity rate of workers in workshop A and B was higher than that in workshop C (χ2= 37, P<0.05), and there was significant difference between male and female（χ2=34.90, P<0.001）. The minimum age of the patients was 18 years old, the maximum age was 48 years old, and the average age was 28 years old. Lactic acid bacteria, Bacillus cereus, Bacteroides fragilis, Enterococcus and Spirillum were detected in all environment and blood samples, and non-specific Legionella gene fragments were detected in some blood samples. The air-conditioning outlet on the ceiling of workshop B had the same homology with the patient's blood samples. Conclusion This is a outbreak of Legionella Pontiaceae fever caused by central air conditioning and humidifier. The source of the outbreak is a pipe air conditioning system.

Clinical epidemiological analysis of 44 cases of brucellosis in Nanning of Guangxi Li Sha, Li Qian, Wei Xiaohong, He Yaoqin, Lu Yibo 2021, 6 (3):  238-241.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.016 Abstract ( 524)    PDF (876KB) ( 537 )    Objective To analyze the clinical epidemiological characteristics of 44 patients with brucellosis in Guangxi, and provide evidence for clinical diagnosis, treatment and prevention. Methods The epidemiology, clinical manifestations, auxiliary examination, treatment and prognosis of 44 cases of brucellosis patients diagnosed by affiliated infectious disease hospital of Nanning, Guangxi Medical University, from 2013 to 2020 were retrospectively analyzed. Results 95.45% of brucellosis patients had contact history with sheep or related products within six months. Fever was the first symptom in 75.00% of patients. Common clinical symptoms and signs included fever (40, 90.91%), chills (14, 31.82%), fatigue (16, 34.36%), sweating (29, 0.45%), digestive symptoms (10, 22.73%), nervous system symptoms (8, 18.18%), muscle soreness (23, 52.27%), joint pain (12, 27.27%), and hepatomegaly or splenomegaly (15, 15.91%). Serum ferritin were elevated in 36 cases of the patients(36, 81.82%). The C-reactive protein level was lightly increased in 30 cases of the patients (30, 68.18%). Conclusion The clinical manifestations and general laboratory indicators of brucellosis lack specificity. Epidemiological history has characteristic significance. Antibacterial treatment was good.

Correlation between CT imaging and the degree of CD4+T lymphocyte reduction of complicated with miliary tuberculosis Ren Rong, Cheng Xi, Liu Rongrong, Yuan Gongling, Liu Jing, Yao Wenyan, Li Min 2021, 6 (3):  242-246.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.017 Abstract ( 377)    PDF (1894KB) ( 504 )    Objective To analyze the correlation between the CT signs of complicated with miliary tuberculosis and the degree of CD4+T lymphocytesreduction, to provide reference for clinical diagnosis and treatment of the disease. Methods Retrospective analysis of CT imaging and clinical characteristics of 49 patients with complicated with miliary tuberculosis admitted to Suzhou Fifth People's Hospital. According to the count of low CD4+T lymphocytes, the patients were divided into groups Ⅰ,Ⅱ, and Ⅲ (respectively 1~50, 51~100, 101~200 cells/μl), with the CT imagines analyzed. Results ①The incidence rate of〝three unevenness〞in the size, density and distribution of miliary nodules in groupⅠwas higher than that in groups Ⅱ and Ⅲ, and the difference was statistically significant (P<0.05). The incidence rate of patchy shadow, pleural effusion, pericardial effusion, mediastinal lymphadenopathy, combined mediastinotomy node tuberculosis and extra-pulmonary tuberculosis in group I were also higher than those in group Ⅱ and Ⅲ, and the differences were statistically significant (P<0.05). ②Among mediastinal lymph node tuberculosis patients, the incidence rate of lymph node enlargement at 2 R, 3 A zone and uneven or separated enhancement in groupⅠ was higher than that in groupⅡ, Ⅲ,the difference was statistically significant (P<0.05). ③Miliary nodules of 3 cases (6.1%) had been absorbed during 3 months, and 46 cases (93.9%) absorbed during 6-12 months or more. Most of them accompanied by vary developed lesions. Conclusion The lower the number of CD4+T lymphocytes, the size, density, and distribution of miliary nodules in CT images of combined with miliary tuberculosis are more uneven, and the incidence rates of mediastinal lymph node tuberculosis, extra-pulmonary tuberculosis, pleural effusion and pericardial effusion are higher.

Epidemiologic characteristics of infectious diarrhea in Shenzhen during 2017-2019 Rao Jian, Yang Rongxing, Mei Shujiang 2021, 6 (3):  247-249.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.018 Abstract ( 434)    PDF (922KB) ( 541 )    Objective To analyze the epidemiological characteristics of infectious diarrhea in Shenzhen,to provide a basis for the formulation of control measures. Methods To use descriptive statistics method to analyze the infectious diarrhea data from 2017 to 2019 collected through National Disease Surveillance System. Results 61 457 cases of infectious diarrhea were reported in Shenzhen from January 2017 to December 2019, which 48 566 (79.02%) were other infectious diarrhea, 12 326 (20.06%) were dysentery and 565 (0.92%) were typhoid fever. The average annual incidence rate was 233.58/100 000, which remained at a high level. The peak seasons of typhoid and bacillary dysentery were summer and autumn. The peak seasons of other infectious diarrhea were autumn and winter. All the districts of Shenzhen had reported infectious diarrhea and the case numbers differed a lot in different districts. The main patients with typhoid were youth at the age of 20 to 39 years;main patients with bacillary dysentery and other infectious diarrhea were children under 10 years old. The top three occupations of patients with infectious diarrhea were preschool children,worker and children in kindergarten and nursery. the main transmission mode of infectious diarrhea in Shenzhen was sporadic, while occasionally outbreak occurred. Conclusion The incidence of infectious diarrhea in Shenzhen remained high level. We should evaluate and improve the infectious diarrhea report system,and strengthen surveillance and control work for infectious diarrhea.

Analysis of the consultation in the department of infectious diseases of Shenzhen People's Hospital Wu Weigang, Huang Qinli, Li Lixian, Wu Xuefang, Yang Zhi, Zeng Furong, Ou Pengcheng, Zeng Xiaobin, Zhou Boping, Wu Shipin, Yang Guilin 2021, 6 (3):  250-254.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.019 Abstract ( 276)    PDF (904KB) ( 501 )    Objective Analyze the disease spectrum of consultation in the Infectious Diseases Department of a general hospital, to explore the demand of general hospitals for the Department of Infectious Diseases. Methods A retrospective study was conducted to analyze the distribution department and consultation characteristics of inpatients who were consulted by the Department of Infectious Disease, Shenzhen People's Hospital from June 2019 to December 2019. Results During this period, a total of 1756 cases of infection department had consultation in our hospital, comes for 4.38% of the total discharge cases in the same period; The first five departments of the consultation cases were 120 cases of gastroenterology (6.83%), 117 cases of Hepatobiliary and Pancreatic Surgery (6.66%), 106 (6.04%), 105 cases of Respiratory and Critical Care Medicine (5.98%), and 104 cases of the Department of Nephrology (5.92%); By disease, infectious diseases 385 cases (21.92%), there are 913 cases of non-infectious infectious diseases (51.99%); In the infectious diseases, most viral hepatitis consultations (343 cases), including 270 (15.38%) of HV and 68 of chronic hepatitis C (3.87%), 42 cases of other infectious diseases (2.39%); In non-communicable infectious diseases, the top five of the consultation were 186 urinary tract infections (10.59%), 148 abdominal infections (8.43%), 146 skin soft tissue infections (8.31%), 139 lung infections (7.92%), and 85 of sepsis (4.84%). Of these, 335 applications (19.08%). Conclusion sThe consultation volume of infectious diseases in general tertiary hospitals is mainly non infectious diseases and infectious diseases, therefore, the grade A general hospitals should strengthen the training of clinicians in the diagnosis and treatment of infectious diseases and the rational use of antibiotics in their specialty.

Review

The application value of new ultrasound technique in cervical lymph node tuberculosis Huang Xiuling, Dong Changfeng 2021, 6 (3):  255-259.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.020 Abstract ( 301)    PDF (883KB) ( 473 )    Tuberculosis is still one of the most deadly diseases today, second only to AIDS, and one of the top 10 diseases that damage people's health.Because of the long incubation period of tuberculosis, most patients with early symptoms are not obvious, easy to be ignored, so as to miss the best time for treatment.In recent years, ultrasound elastography, interventional ultrasound, ultrasonic contrast, super micro-vascular imaging and other new ultrasound technologies have been widely used in clinical practice due to their advantages of simplicity, noninvasive and high efficiency.In addition, ultrasound has a high detection rate of superficial lymph node diseases.Therefore, compared with other imaging examinations, ultrasound can be used as the first choice for the early diagnosis of this disease.In order to better understand the clinical application value of these new ultrasound techniques in cervical lymph node tuberculosis, this article reviews the application value of new ultrasound technique in cervical lymph node tuberculosis.

Research progress on the relationship between multiple vitamins and hepatic carcinoma Tao Zhu, Wang Yan, Lyu Cheng, Guo Hongli, Zhang Fengdan, Zheng Shuang 2021, 6 (3):  260-264.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.021 Abstract ( 525)    PDF (912KB) ( 1003 )    The morbidity and mortality of hepatic carcinoma have been high in China and the world for years.With the improvement of living standards, a dramatically increasing number of citizens are paying attention to vitamins supplementation. Appropriate and rational use of vitamins is particularly important .Each vitamin has different functions. In recent years, multivitamins have been found to have a certain correlation with hepatic carcinoma. Their application in the prevention and treatment of hepatic carcinoma has also received much attention.The detection of vitamins is convenient and fast, which can predict the progression and prognosis of hepatic carcinoma. Vitamins are economically available and have low side effects, which are expected to become newer and more effective anti-hepatic carcinoma drugs. According to the current research progress at home and abroad, this article reviews the relationship between vitamins and hepatic carcinoma.

Education Fields

WHO interpretation of guidelines for COVID-19 considerations for tuberculosis (TB) care Chen Qiuqi, Deng Guofang 2021, 6 (3):  265-269.  DOI: 10.19871/j.cnki.xfcrbzz.2021.03.022 Abstract ( 366)    PDF (1181KB) ( 461 )    For guiding all countries to prevent, control,and reduce the spread of tuberculosis in the COVID-19 pandemic, the World Health Organization experts drew up and issued the COVID-19 considerations for tuberculosis (TB) care. We briefly review the established background, main content, and characteristics of the guideline so that practitioners in the field can better understand the relevant knowledge and improve the ability of prevention and control.